Team:Goettingen/week11-3

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<b>V07_10_2 1<sup>st</sup> round: Ligation</b><br>
<b>V07_10_2 1<sup>st</sup> round: Ligation</b><br>
<ul>
<ul>
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<li>Experiment: <br>The ligation and subsequent clean-up were carried out according to the protocol of <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a>.</li>
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<li>Experiment: <br>The ligation was carried out according to the protocol of <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a> and incubated over night at 16 °C.</li>
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</ul>
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</td></tr>
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<br>
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<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
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<tr bordercolor="black" valign="top">
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<td width="900" bordercolor="black" valign="top">
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<h2><b>V07_11 </b></h2><br>
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<b>V07_11_1 1<sup>st</sup> round: Ethanol precipitation of ligated mutated plasmids</b><br>
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<ul>
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<li>Experiment: <br>The ethanol precipitation was carried out according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>, this time with the right volumes! </li>
</ul>
</ul>
<ul>
<ul>
<li>Observations and results: <br>The corresponding gel showed a band of the expected size.</li>
<li>Observations and results: <br>The corresponding gel showed a band of the expected size.</li>
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</ul><br>
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<b>V07_11_2 1<sup>st</sup> round: Transforamtion of electrocompetent cells wir the mutant plasmid mixture</b><br>
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<ul>
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<li>Experiment: <br>Electrocompetent cells were prepared according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. We transformed 200 µL of cells with 10 µL of DNA according to the protocol of <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a>. Dilution plates and liquid culture was incubated over night at 37 °C.</li>
</ul>
</ul>
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<br></td></tr>
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<br>
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</td></tr>
</table>
</table>
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Revision as of 16:06, 22 September 2012

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#3 Chemoreceptor Library - 11th Week

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V07_10


V07_10_1 1st round: Digestion DpnI/BsaI and clean-up
  • Experiment:
    The digestion and subsequent clean-up were carried out according to the protocol of week 10.
  • Observations and results:
    The corresponding gel showed a band of the expected size.

V07_10_2 1st round: Ligation
  • Experiment:
    The ligation was carried out according to the protocol of week 10 and incubated over night at 16 °C.


V07_11


V07_11_1 1st round: Ethanol precipitation of ligated mutated plasmids
  • Experiment:
    The ethanol precipitation was carried out according to protocol, this time with the right volumes!
  • Observations and results:
    The corresponding gel showed a band of the expected size.

V07_11_2 1st round: Transforamtion of electrocompetent cells wir the mutant plasmid mixture
  • Experiment:
    Electrocompetent cells were prepared according to protocol. We transformed 200 µL of cells with 10 µL of DNA according to the protocol of week 10. Dilution plates and liquid culture was incubated over night at 37 °C.


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