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Team:Trieste/notebook9
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<div id="suicide" class="notebook_section"> | <div id="suicide" class="notebook_section"> | ||
<h2 class="notebook_title">Suicide System</h2> | <h2 class="notebook_title">Suicide System</h2> | ||
| - | + | We loaded the gel with the following amplified T5CumateOperator-RBS_B0034, eluted the fragment and then we tried to cut it with the enzyme, but it did not work as expected, probably because of the restriction enzymes do not work properly at the extremities.</br> | |
| + | </br> | ||
| + | We approached to another strategy: we loaded the gel with the amplified, eluted the fragment and then amplified inside the pGEM vector. In this way, we were able to amplified the fragment with the primer pair SP6 and T7. </br> | ||
| + | </br> | ||
| + | The amplified were then cut with EcoRI/BamHI, heat inactivated and dephosphorylated at the extremities. It worked!!</br> | ||
| + | </br> | ||
| + | We were finally able to clone it upstream the T4 holin (BBa_K112000). | ||
</div> | </div> | ||
<div id="antibody" class="notebook_section"> | <div id="antibody" class="notebook_section"> | ||
Revision as of 16:51, 23 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 9
More
Suicide System
We loaded the gel with the following amplified T5CumateOperator-RBS_B0034, eluted the fragment and then we tried to cut it with the enzyme, but it did not work as expected, probably because of the restriction enzymes do not work properly at the extremities. We approached to another strategy: we loaded the gel with the amplified, eluted the fragment and then amplified inside the pGEM vector. In this way, we were able to amplified the fragment with the primer pair SP6 and T7. The amplified were then cut with EcoRI/BamHI, heat inactivated and dephosphorylated at the extremities. It worked!! We were finally able to clone it upstream the T4 holin (BBa_K112000).Antibody
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Chassis
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
