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Team:WashU/Protocols/PCR
From 2012.igem.org
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==Set up PCR reaction tube== | ==Set up PCR reaction tube== | ||
| - | + | #1uL Template | |
| - | + | #1uL 25mM dNTP's | |
| - | + | #10uL Buffer | |
| - | + | #0.5uL Forward Primer | |
| - | + | #0.5uL Reverse Primer | |
| - | + | #0.5uL TAQ polymerase | |
| - | + | #36.5uL diH2O | |
==Amplification reaction in thermocycler== | ==Amplification reaction in thermocycler== | ||
| - | + | #1 cycle 94 degrees (3 minutes) | |
| - | + | #40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute) | |
==Source== | ==Source== | ||
Thanks to UW iGEM '11 for the above instructions. The original source can be found here: [https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis University Washington gel electrophoresis protocol] | Thanks to UW iGEM '11 for the above instructions. The original source can be found here: [https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis University Washington gel electrophoresis protocol] | ||
Revision as of 17:12, 18 June 2012
