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<!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | ||
| - | + | 1. Download the following spreadsheet : [[File:Team-EPF-Lausanne Ligation.xls]] | |
| - | + | 2. Fill in the pink areas with the vector and fragment concentration, their size and the ratio. | |
| - | + | 3. Add all the suggested ingradients in a microcentrifuge tube, in the order they appear. | |
| - | + | 4. Ligate for 2 hours at 14ºC. | |
| - | + | 5. Inmediatly transform competent bacteria with the ligation product. | |
{{:Team:EPF-Lausanne/Template/ProtocolFooter}} | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} | ||
<noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | ||
1. Download the following spreadsheet : File:Team-EPF-Lausanne Ligation.xls
2. Fill in the pink areas with the vector and fragment concentration, their size and the ratio.
3. Add all the suggested ingradients in a microcentrifuge tube, in the order they appear.
4. Ligate for 2 hours at 14ºC.
5. Inmediatly transform competent bacteria with the ligation product.
"
