Experiment: PCR was performed over night with the Tar forward and reverse primers using Pfu polymerase
and the isolated genomic DNA from DH10B as template according to protocol.
V05_10
Amplification of tar gene
Experiment: PCR was controlled by running a 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
Observations & Results:
A band at about 1.6 kb was detected. Amplification of tar was successful.
V05_11
Amplification of tar gene
Experiment: PCR clean-up using peqGOLD Gel Extraction Kit according to the manual.
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