Team:Wisconsin-Madison

From 2012.igem.org

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       <p align="left" class="classtheinlinecontent"> <strong style="font-style:italic; color: rgb(183, 1, 1);">TCC</strong>
       <p align="left" class="classtheinlinecontent"> <strong style="font-style:italic; color: rgb(183, 1, 1);">TCC</strong>
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       <p align="left" class="classtheinlinecontent">In synthetic biology, one of the most powerful tools a research has is the ability to express heterologous genes in Escherichia coli. Unfortunately this does not always work. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion compatible system for diagnosing the failure of a gene expression at the translational level. This system couples the translation of the target gene to a fluorescent reporter gene.  Fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibiodies or analytical instruments.</p>
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       <p align="left" class="classtheinlinecontent"></p>
<p align="left" class="classtheinlinecontent" style="font-style:italic">This tool is based on a phenomenon known as translational coupling. </p>
<p align="left" class="classtheinlinecontent" style="font-style:italic">This tool is based on a phenomenon known as translational coupling. </p>
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       <p align="right" class="classtheinlinecontent"> <strong style="font-style:italic; color: rgb(183, 1, 1);">Translational couplding cassette</strong></p>
       <p align="right" class="classtheinlinecontent"> <strong style="font-style:italic; color: rgb(183, 1, 1);">Translational couplding cassette</strong></p>
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      In synthetic biology, one of the most powerful tools a research has is the ability to express heterologous genes in Escherichia coli. Unfortunately this does not always work. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion compatible system for diagnosing the failure of a gene expression at the translational level. This system couples the translation of the target gene to a fluorescent reporter gene.  Fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibiodies or analytical instruments.
        
        
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Revision as of 15:13, 23 September 2012


Engineering the limonene production metabolic pathway: TCC.

Creating a tool to evaluate the translation of heterologous genes in Eschericia coli.



TCC

This tool is based on a phenomenon known as translational coupling.






Translational couplding cassette


In synthetic biology, one of the most powerful tools a research has is the ability to express heterologous genes in Escherichia coli. Unfortunately this does not always work. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion compatible system for diagnosing the failure of a gene expression at the translational level. This system couples the translation of the target gene to a fluorescent reporter gene. Fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibiodies or analytical instruments.

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