"
Team:TMU-Tokyo/Notebook/Assay
From 2012.igem.org
(Difference between revisions)
| Line 60: | Line 60: | ||
<Br> | <Br> | ||
<p class="description"> | <p class="description"> | ||
| - | ■Assay1(Device1)<Br></p> | + | <b>■Assay1(Device1)</b><Br></p> |
<Br> | <Br> | ||
<p class="description"> | <p class="description"> | ||
| - | ■Assay2(Device2)<Br></p> | + | <b>■Assay2(Device2)</b><Br></p> |
<Br> | <Br> | ||
<p class="description"> | <p class="description"> | ||
| - | ■Assay3(Device3)<Br></p> | + | <b>■Assay3(Device3)</b><Br> |
| + | FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ | ||
| + | at 340nm.</p> | ||
| + | |||
<Br> | <Br> | ||
Revision as of 19:44, 25 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
Plasmid DNA Pure
Genome DNA Purification
Restruction Purification
DNA Fragment Ligation
Transformation
Electrophoresis
LB Medium
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Assay
■Assay1(Device1)
■Assay2(Device2)
■Assay3(Device3)
FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺
at 340nm.
