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Team:Macquarie Australia/Protocols/ligations
From 2012.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
<tr><td>Downstream Digestion part</td> | <tr><td>Downstream Digestion part</td> | ||
<td>2 µL</td></tr> | <td>2 µL</td></tr> | ||
| - | <tr>< | + | <tr><td>Destination Plasmid</td> |
<td>2 µL</td></tr> | <td>2 µL</td></tr> | ||
<tr><td>10X T4 DNA ligase buffer</td> | <tr><td>10X T4 DNA ligase buffer</td> | ||
Revision as of 00:13, 21 September 2012
Ligation Procedure
A reaction mixture was prepared containing,
| Element | Volume |
| Upstream Digestion part | 2 µL |
| Downstream Digestion part | 2 µL |
| Destination Plasmid | 2 µL |
| 10X T4 DNA ligase buffer | 2 µL |
| T4 DNA ligase | 1 µL |
| H2O | 11 µL |
