Team:Macquarie Australia/Protocols/ligations

From 2012.igem.org

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{{:Team:Macquarie_Australia/Template/MQ12}}
{{:Team:Macquarie_Australia/Template/MQ12}}
<center><h1>Ligation Procedure</h1></center>
<center><h1>Ligation Procedure</h1></center>
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<p>A reaction mixture was prepared containing,<br>
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<center><table border="3" cellpadding="4" cellspacing="0">
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<tr><td>Element</td>
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<td>Volume</td></tr>
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<tr><td>Upstream Digestion part</td>
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<td>2 &micro;L</td></tr>
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<tr><td>Downstream Digestion part</td>
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<td>2 &micro;L</td></tr>
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<tr><tr>Destination Plasmid</td>
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<td>2 &micro;L</td></tr>
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<tr><td>10X T4 DNA ligase buffer</td>
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<td>2 &micro;L</tr></td>
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<tr><td>T4 DNA ligase</td>
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<td>1 &micro;L</td></tr>
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<tr><td>H<sub>2</sub>O</td>
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<td>11 &micro;L</td></tr>
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</table></center>
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</html>

Revision as of 00:12, 21 September 2012



Ligation Procedure

A reaction mixture was prepared containing,

Destination Plasmid</td> <td>2 µL</td> <tr><td>10X T4 DNA ligase buffer</td> <td>2 µL</tr></td> <tr><td>T4 DNA ligase</td> <td>1 µL</td></tr> <tr><td>H2O</td> <td>11 µL</td></tr> </table></center> </html>
Retrieved from "http://2012.igem.org/Team:Macquarie_Australia/Protocols/ligations"
Element Volume
Upstream Digestion part 2 µL
Downstream Digestion part 2 µL