cell-cell communication

Cell-Cell communication Project OVERVIEW

Gene circuit

In this project, we define the signal which E.coli gives out as the romantic feeling of Romeo and Juliet. The story which we reproduce is divided into four scenes.

First, in positive feedback circuit, there are two types of E. coli, Cell R(Romeo) and Cell J(Juliet). In cell R (fig2), production of LasI is stimulated by LuxR/3OC6HSL complex and In cell J (fig2), production of LuxI is stimulated by LasR/3OC12HSL complex. This indicates that 3OC12HSL produced by cell R and 3OC6HSL produced by cell J indirectly stimulate each other’s production. We express the story of "Romeo met Juliet and fell in love instantly." by using "positive feedback circuit" as love which burns vigorously.










Second, "band detect system(fig3)" is a mechanism which controls production of a signal in Cell J body, when the amount of secretion of the signal by Cell R reaches more than fixed. We express the story of "The priest proposes she fake death and wake up 24 hours later.", using a "band detect system" as Juliette's suspended animation .










Third, LacI controls a suicide gene. If Cell R detects reduction of the signal from Cell J, suicide gene separate in Cell R body. As a result, suicide gene is revealed and the number of individuals of Cell R becomes fewer. Reduction of individuals means reduction of the signal. We express the story of "Romeo took the poison." by using a suicide mechanism as suicide of Romeo.










Forth, by the same system as Cell R, suicide gene is revealed and the number of individuals of Cell R becomes fewer. Reduction of individuals means reduction of the signal. We express the story of "Juliet stabbed herself with the dirk." by using a suicide mechanism as suicide of Juliette.

Finaly, we argued about feasibility by performing a simulation using modeling about whether all the above stories are materialized.

Result

In order to make the story of "Romeo and Juliet", we constructed and characterized several important parts and devices. First we characterized (Parts name), both of which are existing parts. Second, we designed and characterized a new (parts name). Third, we designed and characterized a NEW BioBrick device (Parts name),(explain). Forth, we confirmed that (mechanism). Finally, we simulated our project and confirmed the feasibility of our system.

Scene1 Fall in love Positive feedback mechanism

LasR generator

Text... see more

3OC6HSL→pLuxLasI-PLas

Text... see more

3OC12HSL→pLasLuXI-PLux

Text... see more

positive feedback assay

Band detect system

Lux-Tet hybrid promoter assay

Text... see more

Scene3 Romeo Suicide

Las→Lac assay

Scene4 Juliet Suicide

Lux→Lac assay

Text... see more

modeling

Text







>

Conclusion

We succeeded in constructing (number) new BioBrick, (name). We characterized mechanism. From the experimental data and simulation result, we confirmed the feasibility of the project "Romeo and Juliette".