Team:Goettingen/Project/Materials

From 2012.igem.org

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{{GoettingenHeader}}
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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en">
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<meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
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<body class="mediawiki ns-0 ltr page-Team_Goettingen">
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a:hover breaks li:hover in FF2.
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                Keep it sync with the side menu "effective border" width. */
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                            Keep it sync with the drop-down menu width.
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                            Use MenuTailor.css to customize. */
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}
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div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
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div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
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                            Use MenuTailor.css to customize. */
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  font: 12px Verdana; /* Required for IE55. */
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  left: 13em; /* Places the side menu to the right of the drop-down menu.
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                            Keep it sync with the drop-down menu width.
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                            Use MenuTailor.css to customize. */
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}
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div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
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  font: 12px Verdana; /* Required for IE55. */
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-
  left: 13em; /* Places the side menu to the right of the drop-down menu.
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                            Keep it sync with the drop-down menu width.
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                            Use MenuTailor.css to customize. */
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-
}
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/*----------------------------------------------------------------------------- Browser Specific */
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* html div.MenuBar ul li a {
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  float: left; /* Required for IE55 and IE6.
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                            Breaks O9.
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                            Hidden (* html) from non-IE browsers. */
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}
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* html ul.DropDownMenu li a:hover {
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  cursor: hand; /* Required for IE55.
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                  Hidden (* html) from non-IE browsers. */
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}
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ul.DropDownMenu li a:hover {
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                      Hidding it (* html) from non-IE browsers breaks IE7!
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}
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* html div.MenuBar a:hover {
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  text-decoration: none; /* Required for IE55 and IE6.
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                            Hidden (* html) from non-IE browsers. */
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}
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* html div.MenuBar ul li table,
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* html div.MenuBar ul li table td {
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                Hidden (* html) from non-IE browsers. */
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div.MenuBar ul li:hover ul.DropDownMenu,
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div.MenuBar ul li:hover ul.DropDownMenu li a,
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div.MenuBar ul li a:hover ul.DropDownMenu li a {
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                            Sets the drop-down menu "effective background" color. */
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  color: MenuText;
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div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
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div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
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div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
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div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
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  background-color: Menu; /* Side menu unselected items.
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                            Sets the side menu "effective background" color. */
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-
  color: MenuText;
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-
}
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div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
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div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
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  background-color: Highlight; /* Side menu selected item. */
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  color: HighlightText;
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}
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/*-----------------------------------------------------------------------------------------------*/
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/*-------------------------------------------------------------------------------------- General */
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body {
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  background: white;
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  color: black;
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  margin: 0;
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  border: 0;
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  padding: 0;
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}
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div.MenuBar {
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  font: 13px arial, helvetica, sans-serif;
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div.MenuBar ul {
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}
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/*--------------------------------------------------------------------------------------- Colors */
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div.MenuBar {
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div.MenuBar a,
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div.MenuBar ul li:hover ul.DropDownMenu li a,
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div.MenuBar ul li a:hover ul.DropDownMenu li a,
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div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
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div.MenuBar ul li:hover a,
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div.MenuBar ul li a:hover,
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div.MenuBar ul li:hover ul.DropDownMenu,
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div.MenuBar ul li a:hover ul.DropDownMenu,
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div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
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div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
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  background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
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-
}
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/*--------------------------------------------------------------------------------------- Widths */
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/*
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/*
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Menu Bar 1
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Drop-Down Menu #2
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*/
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div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
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div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
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  width: 14em; /* Drop-down menu width. */
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}
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/*...............................................................................................*/
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/*
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Menu Bar 1
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Drop-Down Menu #2
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Side Menu #1
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*/
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
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-
  left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
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                            Keep it sync with the drop-down menu width. */
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-
}
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
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  width: 15em; /* Side menu width. */
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-
}
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-
/*...............................................................................................*/
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/*
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Menu Bar 1
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Drop-Down Menu #2
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Side Menu #2
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*/
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
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-
  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
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                            Keep it sync with the drop-down menu width. */
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-
}
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
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-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
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-
  width: 7em; /* Side menu width. */
+
-
}
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-
/*...............................................................................................*/
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-
/*
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-
Menu Bar 1
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-
Drop-Down Menu #2
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-
Side Menu #3
+
-
*/
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-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
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div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
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-
  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width. */
+
-
}
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
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div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
+
-
  width: 17em; /* Side menu width. */
+
-
}
+
-
/*...............................................................................................*/
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-
 
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</style>
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</p><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
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                <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
+
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                                <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
+
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                <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
+
-
                <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
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Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English <br>
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<table id="toc" class="toc"><tbody><tr><td><div id="toctitle"><h2>Contents</h2> <span class="toctoggle"><a href="javascript:toggleToc()" class="internal" id="togglelink"></a></span></div>
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<li class="toclevel-1"><a href="#Recipes"><span class="tocnumber">1</span> <span class="toctext">Recipes</span></a></li>
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frozen in N<sub>2</sub> or dry ice. Then, cultures were stored at -80°C. Do NOT forget to fill in the according GMO S1 construction sheet!
frozen in N<sub>2</sub> or dry ice. Then, cultures were stored at -80°C. Do NOT forget to fill in the according GMO S1 construction sheet!
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Revision as of 14:17, 20 September 2012

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Recipes

Media


Recipes for liquid and solid media for bacterial cultivation.
  1. LB-Medium
    2. 1% (w/v) bacto tryptone
    0.5% (w/v) bacto yeast extract
    1% (w/v) NaCl
    if required: ad 1% (w/v) agar

  2. M9-Medium

  3. 0.3% Tryptone swimming agar
    4. 1% (w/v) bacto tryptone
    0.5% (w/v) NaCl
    0.3% (w/v) agar


Antibiotics


Stock solutions and concentrations of antibiotics.


Supplements


Stock solutions and concentrations of supplements.


Buffer and Solution


Recipes for stocks of buffers and solutions.
  1. 5x M9 salts stock solution
    2. Stir until dissolved; sterilize by autoclaving at 121°C.
    Amount Substance
    64 g Na2HPO4 × 7 H2O
    15 g KH2PO4
    2.5 g NaCl
    5.0 g NH4Cl
    ad 1000 ml ddH2O


  2. CaCl2 Buffer for competent cells
    3. Stir until dissolved; sterilize by sterile filtration.
    Amount Substance Notes
    7.351 g CaCl2 × 7 H2O Alternative 0.1 M CaCl2.
    15% Glycerol
    ad 500 ml ddH2O


  3. 50x TAE for agarose gels
    4. Stir until dissolved. Working concentration is 1x (40 ml of 50x stock ad 2 l ddH2O).
    Amount Substance
    242 g Tris base
    57.1 ml Glacial acetic acid
    18.6 g EDTA
    ad 1000 ml ddH2O


E. coli

Strains


E. coli strain list - List of the working E. coli strains including genotypes, genetic markers, and alleles.


Storage


Storage of E. coli strains - How to keep E. coli strains.
  1. Glycerol Stock
    • 900 µl over night culture in LB-liquid medium
    • 900 µl 50% sterile glycerol
    Glycerol is extremely viscous. Hence, use cut off tips to pipette. Glycerol stocks were vortexed to ensure uniformly mixture and shock frozen in N2 or dry ice. Then, cultures were stored at -80°C. Do NOT forget to fill in the according GMO S1 construction sheet!


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