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Team:Cambridge/Protocols/biobrick protocols
From 2012.igem.org
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[http://partsregistry.org/Help:Protocols/Linearized_Plasmid_Backbones Linearised Plasmid Backbone] | [http://partsregistry.org/Help:Protocols/Linearized_Plasmid_Backbones Linearised Plasmid Backbone] | ||
| - | [http://partsregistry.org/Help:Learn | + | [http://partsregistry.org/Help:Learn BioBrick standards] Resources from the Registry of Standard Parts |
[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication Biobrick assembly] Resources from openwetware.org | [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication Biobrick assembly] Resources from openwetware.org | ||
Revision as of 17:00, 18 September 2012
BioBricks
[http://partsregistry.org/Help:Distribution_Kits Distribution Kit page] A link to the Registry of Standard Biological Parts
Resuspension of BioBricks from the distribution
see [http://partsregistry.org/Help:Distribution_Kits#Using_the_Spring_2012_DNA_Distribution Using the Spring 2012 DNA Distribution] for a more detailed protocol!
- Locate your BioBrick of interest in the Distribution: [http://partsregistry.org/assembly/libraries.cgi?id=42 Spring 2012 Distribution, DNA Part Repositories] Make sure the plate is properly orientated! The two notched corners of the plate are oriented at the BOTTOM of the plate.
- Pierce foil of required well and pipette 10µL dH2O (distilled water).
- Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes.
- The BioBrick is now ready for transformation
Making Biobricks
[http://partsregistry.org/Help:Protocols/Linearized_Plasmid_Backbones Linearised Plasmid Backbone]
[http://partsregistry.org/Help:Learn BioBrick standards] Resources from the Registry of Standard Parts
[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication Biobrick assembly] Resources from openwetware.org
