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Revision as of 20:22, 13 June 2012 (view source) Xu740 (Talk | contribs) ← Older edit		Revision as of 20:24, 13 June 2012 (view source) Xu740 (Talk | contribs) Newer edit →
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	##1uL 25mM dNTP's		##1uL 25mM dNTP's
	##10uL Phusion HF Buffer		##10uL Phusion HF Buffer
-	##0.5uL Forward Primer (Tm XX <sup>o</sup>C)*	+	##0.5uL Forward Primer
-	##0.5uL Reverse Primer (Tm YY <sup>o</sup>C)*	+	##0.5uL Reverse Primer
	##0.5uL TAQ polymerase		##0.5uL TAQ polymerase
	##36.5uL diH2O		##36.5uL diH2O
-	#Amplification reaction in thermocycler depends	+	#Amplification reaction in thermocycler
		+	##1 cycle 94 degrees (3 minutes)
		+	##40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)

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Revision as of 20:24, 13 June 2012

General PCR Protocol

1. Set up PCR reaction tube
   1. 1uL Template
   2. 1uL 25mM dNTP's
   3. 10uL Phusion HF Buffer
   4. 0.5uL Forward Primer
   5. 0.5uL Reverse Primer
   6. 0.5uL TAQ polymerase
   7. 36.5uL diH2O

1. Amplification reaction in thermocycler
   1. 1 cycle 94 degrees (3 minutes)
   2. 40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)