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Team:Evry/auxin detection
From 2012.igem.org
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That’s the aim of this model that will link the quantity of auxin transported into the cell to GFP degradation that we can observe in our tadpole’s cells. | That’s the aim of this model that will link the quantity of auxin transported into the cell to GFP degradation that we can observe in our tadpole’s cells. | ||
As for us, this model will also help our biologists to find the conditions upon which the reception can work and the help them guess the reasons of possible dysfunction in the auxin reception. | As for us, this model will also help our biologists to find the conditions upon which the reception can work and the help them guess the reasons of possible dysfunction in the auxin reception. | ||
| + | |||
| + | This is what's happening during auxin detection: | ||
| + | <img src="https://static.igem.org/mediawiki/2012/3/36/Scheme_degrad.png"> | ||
| + | Thus, once TIR 1 ang GFP are produced and auxin has entered the cell, it binds with TIR1 and then this complex degrades GFP. | ||
| + | This is what we're going to model. | ||
| + | |||
<h2> Hypotheses </h2> | <h2> Hypotheses </h2> | ||
The same promoter is used for the creation of GFP and TIR, so the efficiency Gamma for the creation of GFP and TIR1 will be identical. | The same promoter is used for the creation of GFP and TIR, so the efficiency Gamma for the creation of GFP and TIR1 will be identical. | ||
<h2> Descritpion of the model </h2> | <h2> Descritpion of the model </h2> | ||
| + | Chemical equations corresponding to: | ||
| + | <ul> | ||
| + | <it> Creation of auxin-TIR1 complex: </it> | ||
| + | </ul> | ||
<h2> Model's calibration </h2> | <h2> Model's calibration </h2> | ||
<h2> Results </h2> | <h2> Results </h2> | ||
Revision as of 14:14, 23 September 2012
Auxin detection
Overview
Now that we’ve managed to model auxin creation and transport, you may be asking yourself ; great, those guys have done all those models, but how can we link it to what we see ? That’s the aim of this model that will link the quantity of auxin transported into the cell to GFP degradation that we can observe in our tadpole’s cells. As for us, this model will also help our biologists to find the conditions upon which the reception can work and the help them guess the reasons of possible dysfunction in the auxin reception. This is what's happening during auxin detection:
Thus, once TIR 1 ang GFP are produced and auxin has entered the cell, it binds with TIR1 and then this complex degrades GFP.
This is what we're going to model.
