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Team:Cambridge/Protocols/M9Medium
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(Difference between revisions)
(Created page with "==M9 Minimal Medium== ''' M9 salts x5 ''' *64 g Na<sub>2</sub>HPO<sub>4</sub>.7H<sub>2</sub>O *15 g KH<sub>2</sub>PO<sub>4</sub> *2.5 g NaCl *5 g NH<sub>4</sub>Cl dissolved in...") |
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'''Final medium''' | '''Final medium''' | ||
| - | *1x | + | *1x M9 salts |
*2mM MgSO<sub>4</sub> | *2mM MgSO<sub>4</sub> | ||
*0.1mM CaCl<sub>2</sub> | *0.1mM CaCl<sub>2</sub> | ||
*0.4% carbon source (e.g. glycerol, glucose, etc.) | *0.4% carbon source (e.g. glycerol, glucose, etc.) | ||
*In sterile H<sub>2</sub>O | *In sterile H<sub>2</sub>O | ||
Revision as of 10:17, 18 September 2012
M9 Minimal Medium
M9 salts x5
- 64 g Na2HPO4.7H2O
- 15 g KH2PO4
- 2.5 g NaCl
- 5 g NH4Cl
dissolved in deionized water to a final volume of 1 L
Autoclave before use.
Final medium
- 1x M9 salts
- 2mM MgSO4
- 0.1mM CaCl2
- 0.4% carbon source (e.g. glycerol, glucose, etc.)
- In sterile H2O
