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Team:Wageningen UR/Journal/week9
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*Last week's PCR products(1,1H,2,2H,3,3H) were digested, ligated into the linearized pSB1A3 backbone and transformed into our electrocompetent DH5 Alfa cells. | *Last week's PCR products(1,1H,2,2H,3,3H) were digested, ligated into the linearized pSB1A3 backbone and transformed into our electrocompetent DH5 Alfa cells. | ||
| - | Then after transformation, we checked the colonies on the plate, but only few colonies on positive control and sample 1, 1H and 2 plate. | + | Then after transformation, we checked the colonies on the plate, but only few colonies on positive control and sample 1(TuYV coat protein), 1H(TuYV coat protein his-tag) and 2 plate (TuYV coat protein + 1/2 readthrough). |
Friday: | Friday: | ||
| - | *Colonies from 1, 1H and 2 plate were picked and checked by colony PCR. But no bands were showed on the agrose gel | + | *Colonies from 1, 1H and 2 plate were picked and checked by colony PCR. But no bands were showed later on the agrose gel check. |
Written by: Han Yue | Written by: Han Yue | ||
Revision as of 08:33, 20 September 2012
week 9: 25 june - 1 july
Office work
Lab work
Biobrick BBa_B0015 (a terminator) was cloned.
TuYV
Wednesday:
- Last week's PCR products(1,1H,2,2H,3,3H) were digested, ligated into the linearized pSB1A3 backbone and transformed into our electrocompetent DH5 Alfa cells.
Then after transformation, we checked the colonies on the plate, but only few colonies on positive control and sample 1(TuYV coat protein), 1H(TuYV coat protein his-tag) and 2 plate (TuYV coat protein + 1/2 readthrough).
Friday:
- Colonies from 1, 1H and 2 plate were picked and checked by colony PCR. But no bands were showed later on the agrose gel check.
Written by: Han Yue
