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Protocol: TAE
To make 200 ml of %0x TAE :
- Tris base: 78.4 g
- Acetic Acid (100%): 11.4 ml
- EDTA: 20ml 0.5 M
- 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M
Team:EPF-Lausanne/Protocol/TAE
From 2012.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
* Tris base: 78.4 g | * Tris base: 78.4 g | ||
| - | * Acetic Acid (100 %): 11.4 ml | + | * Acetic Acid (100%): 11.4 ml |
* EDTA: 20ml 0.5 M | * EDTA: 20ml 0.5 M | ||
** 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M | ** 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M | ||
Revision as of 02:25, 17 September 2012
