"
Team:Carnegie Mellon
From 2012.igem.org
(Difference between revisions)
JSalazar88 (Talk | contribs) |
Enpederson (Talk | contribs) |
||
| Line 376: | Line 376: | ||
<p>We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, <b>for a variety of purposes </b> such as the following: | <p>We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, <b>for a variety of purposes </b> such as the following: | ||
</p> | </p> | ||
| - | <ol><li> | + | <ol><li> Quantifying translational efficiency <i>in vivo </i> |
| - | </li><li> | + | </li><li> Troubleshooting in expression strains |
| - | </li><li> | + | </li><li> mRNA and protein localization |
| + | </li><li> <i>in vivo</i> transcription rate analysis | ||
| + | </li><li> Determining promoter strength <i> in vivo</i> | ||
| + | </li><li> <i>in vivo</i> mRNA and protein half-lives | ||
</li></ol> | </li></ol> | ||
<p>Our proposed BioBrick is novel, and potentially very useful in practice. | <p>Our proposed BioBrick is novel, and potentially very useful in practice. | ||
Revision as of 15:25, 19 June 2012
Welcome to Carnegie Mellon University 2012 iGEM Team Wiki!
Contents |
Introduction: Motivation
- We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
- We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
- We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.
Abstract/Introduction
Motivation question
Humanistic implications go here
Primary Objective: A Useful BioBrick for Synthetic Biologists
We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, for a variety of purposes such as the following:
- Quantifying translational efficiency in vivo
- Troubleshooting in expression strains
- mRNA and protein localization
- in vivo transcription rate analysis
- Determining promoter strength in vivo
- in vivo mRNA and protein half-lives
Our proposed BioBrick is novel, and potentially very useful in practice.
Secondary Objective: Humanistic Practice
FAQ/Terminology in engineering Escherichia coli to monitor these variables via fluorescence. Find out more about Carnegie Mellon: (CMU Home Page).
Further Considerations
In the pursuit of our project, as well as the biological aspects, we:
- Considered aspects of scale-up, including the ethical, legal and social implications of our BioBrick,
- Programmed a new piece of software for modeling our BioBrick to students,
- Developed and tested techniques for measuring translational efficiency and transcriptional strength,
- Participated in human practices demonstration xxx.
