Team:Penn/Notebook

From 2012.igem.org

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<li>Finalized synthesis order (minus linker)</li>
<li>Finalized synthesis order (minus linker)</li>
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<h2 class="accordion-header">Week 4</h2>
<h2 class="accordion-header">Week 4</h2>
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<p><b>June 22</b></p>
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&nbsp;&nbsp;<p>Wet Lab</p>
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<ul>
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<li>Ashwin - Repeated miniprep on pDawn and did test cut</li>
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<li>Peter  - Miniprepped pet26b and digested with BglII and EcorRI</li>
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<li>Avin - Miniprepped pet26b, made glycerol stock and digested with BamH1 and Not1</li>
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</ul>
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&nbsp;&nbsp;<p>Dry Lab</p>
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<ul>
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<li>Avin - Finalized and sent in synthesis order (still awaiting order confirmation)</li>
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</ul>
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Update for June 25
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Wet Lab
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Ashwin/Avin - Column purification, ligation, and transformation of Pet26b-mCherry
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Mike -
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Peter -run gel of eGFP/plsr ligation
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Nikita -
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Dry Lab
 +
Avin - Sent in final gene synthesis order
 +
Mike - reviewed pDawn protocol, reviewed TetR sequences
 +
Peter- Order restriction enzymes from cell center
 +

Revision as of 10:46, 15 September 2012

Penn 2012 iGEM Wiki

June 2012 Notebook

Week 1

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

  

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

  

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Wet Lab

  • Fill in later....
  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Week 3

June 18th

  

Wet Lab

  • Miniprep pDawn and pDusk
  • Test cut pDawn and pDusk with XmaI, analytical gel was correct
  • Prep cut pDawn and pDusk with BamHI and NotI, gel purified
  

Dry Lab

  • Ordered and picked up PCR purification kit from cell center
  • Additional orders through cell center
  • Designed primers for one of Peter's components (forgot which)

June 20

  

Wet Lab

  • Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
  • PCR purified fragments (Peter), then ran gel?
  

Dry Lab

  • Researched DARPin binding domains and linkers
  • Finalized some biobrick orders
  • Finalized synthesis order (minus linker)

Week 4

June 22

  

Wet Lab

  • Ashwin - Repeated miniprep on pDawn and did test cut
  • Peter - Miniprepped pet26b and digested with BglII and EcorRI
  • Avin - Miniprepped pet26b, made glycerol stock and digested with BamH1 and Not1
  

Dry Lab

  • Avin - Finalized and sent in synthesis order (still awaiting order confirmation)
Update for June 25 Wet Lab Ashwin/Avin - Column purification, ligation, and transformation of Pet26b-mCherry Mike - Peter -run gel of eGFP/plsr ligation Nikita - Dry Lab Avin - Sent in final gene synthesis order Mike - reviewed pDawn protocol, reviewed TetR sequences Peter- Order restriction enzymes from cell center

July 2012 Notebook

Week 5

Week 6

Week 7

Week 8

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