Team:Penn/Notebook

From 2012.igem.org

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<h2 class="accordion-header">Installation</h2>
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<h2 class="accordion-header">Week 3</h2>
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<p class="first-p">First, unzip the "vallenato.zip" file and place the "vallenato" folder in the same directory as the html file(s) that will be using the script. If placed in a different folder, you will need to update the paths below.</p>
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<p>June 18th</p>
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<p>In the &lt;head&gt; section of your html you will need to link to jQuery, the Vallenato script and stylesheet. You can use the following code:</p>
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&nbsp;&nbsp;<p>Wet Lab</p>
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<ul>
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<li>Miniprep pDawn and pDusk</li>
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<p class="first-p">&lt;script src="http://code.jquery.com/jquery-latest.js" type="text/javascript"&gt;&lt;/script&gt;</p>
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<li>Test cut pDawn and pDusk with XmaI, analytical gel was correct</li>
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<li>Prep cut pDawn and pDusk with BamHI and NotI, gel purified</li>
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</ul>
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&nbsp;&nbsp;<p>Dry Lab</p>
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<ul>
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<li>Ordered and picked up PCR purification kit from cell center</li>
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<li>Additional orders through cell center</li>
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<li>Designed primers for one of Peter's components (forgot which)</li>
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</ul>
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<br>
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<p>June 20</p>
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<p>&lt;script src="vallenato/vallenato.js" type="text/javascript"&gt;&lt;/script&gt;</p>
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&nbsp;&nbsp;<p>Wet Lab</p>
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<ul>
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<li>Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan</li>
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<li>PCR purified fragments (Peter), then ran gel?</li>
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</ul>
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<p class="last-p">&lt;link rel="stylesheet" href="vallenato/vallenato.css" type="text/css" media="screen"&gt;</p>
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&nbsp;&nbsp;<p>Dry Lab</p>
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</code>
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</div>
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<li>Researched DARPin binding domains and linkers</li>
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<li>Finalized some biobrick orders</li>
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<li>Finalized synthesis order (minus linker)</li>
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</ul>
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</div>
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Revision as of 10:34, 15 September 2012

Penn 2012 iGEM Wiki

June 2012 Notebook

Week 1

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

  

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

  

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Wet Lab

  • Fill in later....
  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Week 3

June 18th

  

Wet Lab

  • Miniprep pDawn and pDusk
  • Test cut pDawn and pDusk with XmaI, analytical gel was correct
  • Prep cut pDawn and pDusk with BamHI and NotI, gel purified
  

Dry Lab

  • Ordered and picked up PCR purification kit from cell center
  • Additional orders through cell center
  • Designed primers for one of Peter's components (forgot which)

June 20

  

Wet Lab

  • Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
  • PCR purified fragments (Peter), then ran gel?
  

Dry Lab

  • Researched DARPin binding domains and linkers
  • Finalized some biobrick orders
  • Finalized synthesis order (minus linker)

Retrieved from "http://2012.igem.org/Team:Penn/Notebook"