Team:Penn/Notebook

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Revision as of 10:31, 15 September 2012

Penn 2012 iGEM Wiki

June 2012 Notebook

Week 1

June 6th

Set up some lab equipment
Autoclaved for a while
Organized biobrick stuff
Called Vinoo about DNA planning

June 7th

Transformed Cph8, pLsr, and LuxS
Placed order with Vinoo
Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

Wet Lab

PCR'd mCherry from NAS157
Ran 1% Gel and purified product

Dry Lab

Designed primers for LsR promoter
Meeting with Dr. Sarkar

June 12th

Wet Lab

Digested mCherry PCR product with BamHI and NotI
Column purified mCherry and ligated into NAS152 backbone
Transformed NAS152-mCherry into DH5alpha
Poured 25 LB-Kan plates

Dry Lab

Research more information about bacterial drug delivery system
More research into biofilm project

June 14th

Wet Lab

Fill in later....

Dry Lab

Met with Dr. Goulian, obtained pDawn and pDusk
Identified inaK as a surface display gene we can use

Installation

First, unzip the "vallenato.zip" file and place the "vallenato" folder in the same directory as the html file(s) that will be using the script. If placed in a different folder, you will need to update the paths below.

In the <head> section of your html you will need to link to jQuery, the Vallenato script and stylesheet. You can use the following code:


						<script src="http://code.jquery.com/jquery-latest.js" type="text/javascript"></script>

						<script src="vallenato/vallenato.js" type="text/javascript"></script>

						<link rel="stylesheet" href="vallenato/vallenato.css" type="text/css" media="screen">

@@ Line 108: / Line 108: @@
 <!----------------------------------------------------------------------------->
+<p>June 2012 Notebook
 <div id="accordion-container">
 			<h2 class="accordion-header">Week 1</h2>