Team:Penn/Notebook

From 2012.igem.org

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<p><b>June 8th</b></p>
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<p><b>June 11th</b></p>
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                            <p>Wet Lab</p>
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                                                <ul>
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                                                  <li>PCR'd mCherry from NAS157</li>
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                                                  <li>Ran 1% Gel and purified product</li>
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                                                </ul>
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<p>Dry Lab</p>
 +
<ul>
 +
<li>Designed primers for LsR promoter</li>
 +
<li>Meeting with Dr. Sarkar</li>
 +
</ul>
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<p><b>June 12th</b></p>
                             <p>Wet Lab</p>
                             <p>Wet Lab</p>
                                                 <ul>
                                                 <ul>
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<li>More research into biofilm project</li>
<li>More research into biofilm project</li>
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</ul>
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<p><b>June 11th</b></p>
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<p><b>June 14th</b></p>
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<p>Wet Lab</p>
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                                                 <ul>
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                                                   <li>Transformed Cph8, pLsr, and LuxS</li>
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                                                   <li>Fill in later....</li>
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                                                  <li>Placed order with Vinoo</li>
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<li>Developed idea using PGY/PCN system to activate a gene</li>
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                                                 </ul>
                                                 </ul>
 +
<p>Dry Lab</p>
 +
<ul>
 +
<li>Met with Dr. Goulian, obtained pDawn and pDusk</li>
 +
<li>Identified inaK as a surface display gene we can use</li>
 +
</ul>
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Morbi felis libero, porta non, sagittis ac, consectetur in, sem.
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<p>June 18th</p>
 +
 
 +
<p>Wet Lab</p>
 +
<ul>
 +
<li>Miniprep pDawn and pDusk</li>
 +
<li>Test cut pDawn and pDusk with XmaI, analytical gel was correct</li>
 +
<li>Prep cut pDawn and pDusk with BamHI and NotI, gel purified</li>
 +
</ul>
 +
<p>Dry Lab</p>
 +
<ul>
 +
<li>Ordered and picked up PCR purification kit from cell center</li>
 +
<li>Additional orders through cell center</li>
 +
<li>Designed primers for one of Peter's components (forgot which)</li>
 +
</ul>
</div>
</div>
</div>
</div>

Revision as of 09:38, 15 September 2012

Penn 2012 iGEM Wiki

Drug Delivery Notebook

Expand All | Collapse All

  • June 2012

    • Week 1

      June 6th

      • Set up some lab equipment
      • Autoclaved for a while
      • Organized biobrick stuff
      • Called Vinoo about DNA planning

      June 7th

      • Transformed Cph8, pLsr, and LuxS
      • Placed order with Vinoo
      • Developed idea using PGY/PCN system to activate a gene

    • Week 2

      June 11th

      Wet Lab

      • PCR'd mCherry from NAS157
      • Ran 1% Gel and purified product

      Dry Lab

      • Designed primers for LsR promoter
      • Meeting with Dr. Sarkar

      June 12th

      Wet Lab

      • Digested mCherry PCR product with BamHI and NotI
      • Column purified mCherry and ligated into NAS152 backbone
      • Transformed NAS152-mCherry into DH5alpha
      • Poured 25 LB-Kan plates

      Dry Lab

      • Research more information about bacterial drug delivery system
      • More research into biofilm project

      June 14th

      Wet Lab

      • Fill in later....

      Dry Lab

      • Met with Dr. Goulian, obtained pDawn and pDusk
      • Identified inaK as a surface display gene we can use

    • Week 3

      June 18th

      Wet Lab

      • Miniprep pDawn and pDusk
      • Test cut pDawn and pDusk with XmaI, analytical gel was correct
      • Prep cut pDawn and pDusk with BamHI and NotI, gel purified

      Dry Lab

      • Ordered and picked up PCR purification kit from cell center
      • Additional orders through cell center
      • Designed primers for one of Peter's components (forgot which)

    • Week 4

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.
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  • July 2012

    • Week 5

      Donec elementum lobortis lorem. Sed aliquet lacus vitae nibh. Sed ullamcorper pharetra augue. Lorem ipsum dolor sit amet, consectetur adipiscing elit.

    • Week 6

      Vestibulum blandit mauris elementum mauris.

    • Week 7

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.

    • Week 8

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.

  • August 2012

    • Week 9

      Donec elementum lobortis lorem. Sed aliquet lacus vitae nibh. Sed ullamcorper pharetra augue. Lorem ipsum dolor sit amet, consectetur adipiscing elit.

    • Week 10

      Vestibulum blandit mauris elementum mauris.

    • Week 11

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.

    • Week 12

      Morbi felis libero, porta non, sagittis ac, consectetur in, sem.

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