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-	Synechosunshine	+	The Project
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-	<p align="justify">We are an interdisciplinary group from Valencia composed by biotechnology, biology, marine science and engineering students from three different universities. We're actually working in a light producing cyanobacteria system.</p>	+	<p align="justify"><h4>Synechosunshine: photosynthetically powered biolamp</h4>
		+	We present an artificial consortium between 2 specialized bacteria by the means of genetic engineering, in order to obtain a photosynthetically fed biolamp. It is a novel proposal of synthetic ecology, based on the use of an efficient photosynthesizer (the cyanobacterium Synechococcus elongatus) modified to become an exporter of sucrose and diel switch of the activity of Aliivibrio fischeri, a marine bacterium widely known for its bioluminescent properties in response to quorum sensing signals. Our modified cyanobacteria feed the population of A. fischeri through a transporter protein and produce AHL to induce bioluminescence in response to the activity of a photosensitive operator, which would activate only at night. We also have tried to transform different microalgae with bioluminescence genes to test their effectiveness. We look forward to develop an efficient and autosufficient environmentally friendly biolamp, with potential application to cover the illumination needs of many infrastructure sectors.</p>
-	<p align="justify">Our objective is to create an autosufficient biolamp, which will turn on during night without any other input than the absence of light. For that, we are going to modify the cyanobacteria Synechococcus elongatus sp 7942 and the green algae Chlamydomonas reinhardtii placing genes of the lux/luc operon from Vibrio fischeri under the control of different light-regulated promoters, such as psbAI.</p>
-	<p align="justify">Efforts will be done to improve actual light emission intensity limitations to achieve a biological platform for further industrial research.</p>
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Revision as of 14:18, 14 September 2012

The Project

Synechosunshine: photosynthetically powered biolamp

We present an artificial consortium between 2 specialized bacteria by the means of genetic engineering, in order to obtain a photosynthetically fed biolamp. It is a novel proposal of synthetic ecology, based on the use of an efficient photosynthesizer (the cyanobacterium Synechococcus elongatus) modified to become an exporter of sucrose and diel switch of the activity of Aliivibrio fischeri, a marine bacterium widely known for its bioluminescent properties in response to quorum sensing signals. Our modified cyanobacteria feed the population of A. fischeri through a transporter protein and produce AHL to induce bioluminescence in response to the activity of a photosensitive operator, which would activate only at night. We also have tried to transform different microalgae with bioluminescence genes to test their effectiveness. We look forward to develop an efficient and autosufficient environmentally friendly biolamp, with potential application to cover the illumination needs of many infrastructure sectors.

Here is our Synechococcus culture. Everything is working out!!!

Contact us: igemvalencia2012@gmail.com