Team:Goettingen/week7-2

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#2 Speed Improvement - 7th week

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V06_11

V06_11_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_11_2 Chemical retransformartion of the new flhDC-promoter constructs into E. coli (DH10B)

Experiment:
For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:
20G - #1
20G - #2
2G - #1
2G - #2
2G - #3
20I - #2
20I - #3
18O - #2
18O - #3
18K - #1
18K - #2
18K - #3
Observations & Results:
The retransformation was successful since all plates showed numeours colonoes except the negative control.

V06_12

Preparation of over night cultures

Experiment:
In order to gain further plasmid material over night cultures of all eight flhDC-promoter constructs were prepared for subsequent plasmid isolation.
20E - #2
20G - #1
2G - #1
20I - #2
18M - #2
18O - #2
18K - #1
18C - #2

V06_13

V06_08_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_08_2 Chemical transformartion of all flhDC-promoter constructs into E. coli (DH10B)

Experiment:
For the chemical transformation the standard protocol was followed.
Observations & Results:
The transformation was successful since all plates showed numeours colonoes except the negative control.

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@@ Line 570: / Line 570: @@
 <ul>
 <li>Experiment: <br>
-In order to gain further plasmid material over night cultures were prepared for subsequent plasmid isolation.
+In order to gain further plasmid material over night cultures of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br>
+E - #2 <br>
+G - #1 <br>
+G - #1 <br>
+I - #2 <br>
+M - #2 <br>
+O - #2 <br>
+K - #1 <br>
+C - #2 <br>
 <br></td></tr>
 </table>
-<br>
-<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
-<tr bordercolor="black" valign="top">
-<td width="900" bordercolor="black" valign="top">
-<h2><b>V06_07 </b></h2><br>
-<b>Preparation of over night cultures</i></b><br>
-<ul>
-<li>Experiment: <br>
-Over night cultures were preaped of three colonies of each constructs in order to isolate the plasmids.
-<br> </li>
-</ul>
-<br></td></tr>
-</table>
 <br>
@@ Line 597: / Line 587: @@
 <tr bordercolor="black" valign="top">
 <td width="900" bordercolor="black" valign="top">
-<h2><b>V06_08 </b></h2><br>
+<h2><b>V06_13 </b></h2><br>
 <b>V06_08_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>
 <ul>
@@ Line 605: / Line 595: @@
 </ul>
 <br>
-<b>V06_08_2  Test digestion of the new <i>flhDC</i>-promoter constructs</b><br>
+<b>V06_08_2 Chemical transformartion of all <i>flhDC</i>-promoter constructs into E. coli (DH10B)</b><br>
 <ul>
 <li>Experiment:  <br>
-In order to prove correct insertion of <i>flhDC</i> a test digestion was performed using SpeI and XbaI according to the protocol.<br>
+For the chemical transformation the standard protocol was followed. <br>
 <li>Observations & Results: <br>
-For each construct two to three clones hosting the correctly inserted gene in the plasmid could be obtained. Those colonies still containing the rfp gene and were thus rejected.</li>
+The transformation was successful since all plates showed numeours colonoes except the negative control.</li>
 <br></td></tr>
 </table>