Team:Penn/Notebook/060612

From 2012.igem.org

(Difference between revisions)
(→Peter)
 
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*Autoclaved 500 mL LB Broth for scale up of luxS (Plate 3 Well 2H, pSB1A2), plsr (Plate 2 Well 14H in pSB1A2 resistance to Amp)
*Autoclaved 500 mL LB Broth for scale up of luxS (Plate 3 Well 2H, pSB1A2), plsr (Plate 2 Well 14H in pSB1A2 resistance to Amp)
*Added iGEM logo to wiki
*Added iGEM logo to wiki
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==6/7/12==
 
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*Resuspended available biobricks luxS & plsr (resuspended in 10uL ddH<sub>2</sub>O)
 
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*Resuspended DNA transformed into DH5α (40uL DH5α+2uL DNA) & plated on LB plates w/ 15 uL of 100 mg/mL Amp spread on surface
 
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*Requested lsrR & lsrK from iGEM HQ
 
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==6/8/12==
 
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*iGEM Wiki now split into two separate Notebooks
 
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*Plenty of colonies, too many to count(TMTC) grew from plsr & luxS transformation from 6/7/12
 
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**Selected one colony each
 
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**Grew in 10 mL LB+Amp (100 ug/mL)
 
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*Contacted researchers for V. harveyi bioassay & lysostaphin, V. harveyi must be purchased through ATCC ($300), lysostaphin obtained thru MTA.
 
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==6/9/12==
 
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*~2mL of LB evaporated during incubation
 
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*Cells spun down @ 5000 rpm for 10 min
 
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*Miniprepped 4x Lysis into 1 column
 
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*[plsr]=112 ng/uL
 
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*[luxS]=114 ng/uL
 
==Mike==
==Mike==

Latest revision as of 23:45, 9 June 2012

June 6th, 2012

[http://en.wikipedia.org/wiki/Wikipedia:Cheatsheet Wikipedia Cheat Sheet]

Contents

6/6/12

  • Set up equipment
  • Autoclaved 2x 1L Sterile ddH2O
  • Autoclaved 500 mL LB Broth for scale up of luxS (Plate 3 Well 2H, pSB1A2), plsr (Plate 2 Well 14H in pSB1A2 resistance to Amp)
  • Added iGEM logo to wiki

Mike

Ashwin

Nikita

Avin

Retrieved from "http://2012.igem.org/Team:Penn/Notebook/060612"