Team:Bielefeld-Germany/Labjournal/week7

Week 7 (06/11 - 06/17/12)

Monday June 11th

• Team Bacterial Laccases: Prepared plasmids for sequencing. We sent another isolated plasmid with the E. coli CueO laccase. Also the Tth- laccase, CotA (B. halodurans and CotA (B.pumilus) plasmids were ready for sequencing.

Tuesday June 12th

Wednesday June 13th

• Team Modeling: programming our first differential equation and finding the ODE15s function witch solves these equations.

• Team Bacterial Laccases: Since the GC amount of the S. griseus and S. lavendulae laccases are high we used betain to solve the PCR problem. Addition of betain did not changed anything of the result, we still didn't got our laccase DNA.

Thursday June 14th

• Team Activity Tests: Since our Tecan microplate reader is not able to actively cool down to 4 °C we got the chance to meet the photometer Carry. Check "protocols" for further information about her. We used the same set up with 100 mM natrium acetate buffer, 0,1 U T. versicolor laccase and 0,1 mM ABTS as before but now measured at 4°C. Our team is planning to visit a municipal sewage plant for getting some insights into the water conditions there, so we will for sure test other temperatures after having more information. Let´s hope the water there is a little warmer since laccase does not seem to be totally satisfied at 4°C. I would not either.

• Team Bacterial laccase: Because our PCRs have not worked well we thought it may depend on the primer annealing temperature so we did gradient PCR with the same conductions as before (PCR June 4th) . But this also showed as no result. Our next idea was to let the bacteria grow in media and isolate genomic DNA.

Friday June 15th

Saturday June 16th

Sunday June 17th

Sunday

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