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Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
(Difference between revisions)
(→ZombiE.coli parts) |
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|- | |- | ||
| pReporter | | pReporter | ||
| - | | BBa_I714891 | + | | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] |
| GFP: Green fluorescent protein (''fused with SDY... is there a different part we should use?'') | | GFP: Green fluorescent protein (''fused with SDY... is there a different part we should use?'') | ||
| [[File:BBa_I714891.fasta.txt]] | | [[File:BBa_I714891.fasta.txt]] | ||
| Line 84: | Line 84: | ||
|- | |- | ||
| pReporter | | pReporter | ||
| - | | BBa_J06504 | + | | [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] |
| Monomeric RFP optimized for bacteria | | Monomeric RFP optimized for bacteria | ||
| [[File:BBa_J06504.fasta.txt]] | | [[File:BBa_J06504.fasta.txt]] | ||
| Line 90: | Line 90: | ||
|- | |- | ||
| pReporter | | pReporter | ||
| - | | BBa_K081009 | + | | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] |
| LasI protein generator (TERM-) | | LasI protein generator (TERM-) | ||
| [[File:BBa S03967.fasta.txt]] | | [[File:BBa S03967.fasta.txt]] | ||
| 2012 kit plate 2 (10N) | | 2012 kit plate 2 (10N) | ||
|} | |} | ||
Revision as of 21:50, 9 June 2012
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box | Slot | Name | Description | Source |
|---|---|---|---|---|
| iGEM 2012 Plasmid Box 1 | 2 | cadA | ASKA cadA plasmid | Barrick Lab |
| iGEM 2012 Plasmid Box 1 | 3 | ldcC | ASKA ldcC plasmid | Barrick Lab |
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box | Slot | Name | Description | Source |
|---|---|---|---|---|
| iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab |
| iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab |
| iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab |
| iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab |
| iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab |
ZombiE.coli parts
| Plasmid | Part | Description | Sequence | Source |
|---|---|---|---|---|
| pReporter | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) | File:BBa I714891.fasta.txt | 2012 kit plate 2 (15L) |
| pReporter | [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] | Monomeric RFP optimized for bacteria | File:BBa J06504.fasta.txt | 2012 kit plate 1 (13F) |
| pReporter | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | LasI protein generator (TERM-) | File:BBa S03967.fasta.txt | 2012 kit plate 2 (10N) |
