Team:Chalmers-Gothenburg/Results

From 2012.igem.org

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=='''Deletion of ''CWP2'' gene'''==
=='''Deletion of ''CWP2'' gene'''==
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'''Gene Deletion'''
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<!--One task of our iGEM project was the deletion of the ''CWP2'' gene, which is encoding a cell wall mannoprotein. By removing it, we aimed for higher cell wall permeability and thus higher chances of our ligand to pass the cell wall and to bind to the membrane-bound receptor.
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One task of our iGEM project was the deletion of the ''CWP2'' gene, which is encoding a cell wall mannoprotein. By removing it, we aimed for higher cell wall permeability and thus higher chances of our ligand to pass the cell wall and to bind to the membrane-bound receptor.
The gene deletion was performed according to the bipartite method.  The results from the first PCR reactions, where we amplified the overlapping fragments, can be seen in Figure 3.  
The gene deletion was performed according to the bipartite method.  The results from the first PCR reactions, where we amplified the overlapping fragments, can be seen in Figure 3.  
[[File:Resultpcr1.jpg|thumb|600px|center|Figure 3: A) A schematic illustration of the first four PCR reactions. The 5' and 3' end of the kanMX (kanamycin resistance) cassette, flanked to loxP sites and available in vectors, are amplified by PCR (PCR reaction 1 and 2). In addition, 500 bp of the up- and downstream sequence of the CWP2 gene are amplified from genomic DNA using primers with 5' extensions that are complement to the loxP sites as indicated with lines in the schematic illustration above. B) The results of the four PCR reactions on gel. The expected sizes of the kanMX and CWP2 fragments are 1000 bp and 500 bp respectively which correspond to the sizes observed on the gel.]]
[[File:Resultpcr1.jpg|thumb|600px|center|Figure 3: A) A schematic illustration of the first four PCR reactions. The 5' and 3' end of the kanMX (kanamycin resistance) cassette, flanked to loxP sites and available in vectors, are amplified by PCR (PCR reaction 1 and 2). In addition, 500 bp of the up- and downstream sequence of the CWP2 gene are amplified from genomic DNA using primers with 5' extensions that are complement to the loxP sites as indicated with lines in the schematic illustration above. B) The results of the four PCR reactions on gel. The expected sizes of the kanMX and CWP2 fragments are 1000 bp and 500 bp respectively which correspond to the sizes observed on the gel.]]
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{{Team:Chalmers-Gothenburg/footer}}
{{Team:Chalmers-Gothenburg/footer}}

Revision as of 18:01, 13 September 2012

Sponsors


Microsynth


GATC Biotech


Chalmers


Mölnlycke Health Care

Contents

Results Summary

Survival of yeast in urine

Deletion of CWP2 gene

Expression of human LH/CG receptor

Introduction of indigo synthesizing genes











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