Team:Wageningen UR/Protocol

From 2012.igem.org

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<li>[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]</li>
<li>[[Team:Wageningen_UR/Protocol/Reassemblybuffer|Reassembly buffer]]</li>
<li>[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]</li>
<li>[[Team:Wageningen_UR/Protocol/Virusbuffer|Virus buffer]]</li>
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<li>[[Team:Wageningen_UR/Protocol/FormationBufferHepB|Formation Buffer HepBcAg]]</li>
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<li>[[Team:Wageningen_UR/Protocol/WashingBuffer|Washing Buffer HepBcAg]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer1x|Towbin's electrotransfer buffer 1x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li>
<li>[[Team:Wageningen_UR/Protocol/Towbinselectrotransferbuffer10x|Towbin's electrotransfer buffer 10x]]</li>

Revision as of 11:51, 13 September 2012

Contents

Methods

The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.

  • The production of our VLPs monomers is done in E.coli, while the formation of our VLPs is done in vitro. We used multiple techniques to make this happen. Click here to read more
  • After the production of our VLPs we have our formed VLPs, but this product is not pure enough. So we developed a two step method to purify the product. Click here to read more

Protocol

Medium & Buffer recipes

CCMV Coat Protein VLP formation

Hepatitis B Coat Protein VLP formation

TuYV Coat Protein VLP formation

PLRV Coat Protein VLP formation

General Protocol

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