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Team:TU Darmstadt/Materials/T4 DNA Ligase
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Latest revision as of 20:45, 26 September 2012
Contents |
T4 DNA Ligase
About
T4 DNA ligase is an enzyme that facilitates the joining of DNA stands by catalyzing phosphodiester bonds. It was isolated from [http://en.wikipedia.org/wiki/Enterobacteria_phage_T4 bacteriophage T4] and is used to generate recombinant DNA sequences.[1]
Working conditions
For ligating extended oligos in a PCR reaction it is necessary to have phosphorilated 5'-ends. Oligos will only be ligated when they are assembled on a compementary strand. [2]
Supplier
- NEB http://www.neb.com/nebecomm/products/productM0202.asp
References
[1] http://en.wikipedia.org/wiki/DNA_ligase
[2] http://openwetware.org/wiki/Thermostable_DNA_ligase
