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Team:TU Darmstadt/Materials/T4 DNA Ligase
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For ligating extended oligos in a PCR reaction it is necessary to have [https://2012.igem.org/Team:TU_Darmstadt/Materials#Phosphatases phosphorilated] 5'-ends. Oligos will only be ligated when they are assembled on a compementary strand. <sup>[2]</sup> | For ligating extended oligos in a PCR reaction it is necessary to have [https://2012.igem.org/Team:TU_Darmstadt/Materials#Phosphatases phosphorilated] 5'-ends. Oligos will only be ligated when they are assembled on a compementary strand. <sup>[2]</sup> | ||
=== Supplier === | === Supplier === | ||
| - | * NEB http://www.neb.com/nebecomm/products/ | + | * NEB http://www.neb.com/nebecomm/products/productM0202.asp |
| + | |||
=== References === | === References === | ||
[1] http://en.wikipedia.org/wiki/DNA_ligase | [1] http://en.wikipedia.org/wiki/DNA_ligase | ||
[2] http://openwetware.org/wiki/Thermostable_DNA_ligase | [2] http://openwetware.org/wiki/Thermostable_DNA_ligase | ||
Revision as of 19:26, 11 September 2012
Contents |
T4 DNA Ligase
About
T4 DNA ligase is an enzyme that facilitates the joining of DNA stands by catalyzing phosphodiester bonds. It was isolated from [http://en.wikipedia.org/wiki/Enterobacteria_phage_T4 bacteriophage T4] and is used to generate recombinant DNA sequences.[1]
Working conditions
For ligating extended oligos in a PCR reaction it is necessary to have phosphorilated 5'-ends. Oligos will only be ligated when they are assembled on a compementary strand. [2]
Supplier
- NEB http://www.neb.com/nebecomm/products/productM0202.asp
References
[1] http://en.wikipedia.org/wiki/DNA_ligase
[2] http://openwetware.org/wiki/Thermostable_DNA_ligase
