Team:Uppsala University/Results

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<h2>sRNA gene silencing</h2>
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<p>We have developed a modular screening system and protocol for finding silencing sRNA:s against arbitrary genes. Using this, we have found a strongly silencing sRNA:s against a clinical antibiotic gene and lowered the minimary inhibatory concentration five-fold in resistant bacteria.
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<tr><td class="subtext"><h2>DNA level - Plasmid removal</h2></td>
 
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<p><a href="https://2012.igem.org/Team:Uppsala_University/sRNA"> Read more...</a></p>
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<h2>New standard backbone series</h2>
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<p>We have constructed a range of new standard backbones, especially low copy backbones and backbones with built-in lacIq repression for tight control of toxic genes. This work was induced as it turned out that the common registry pSB4 backbones all have a faulty copy number regulation, while we needed low copy backbones for out project. <a href="https://2012.igem.org/Team:Uppsala_University/Backbones"> Read more...</a></p>
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<tr><td class="subtext"><h2>Transcriptional level - Targeting gene networks</h2></td>
 
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<tr><td class="subtext"><h2>Translational level - Silencing sRNA:s</h2></td>
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<h2>Promoter characterization</h2>
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<p id="second">We have characterized promoter strengths of ten popular promoters with Fluorescense Activated Cell Sorting (FACS), putting syntetic and wild-type promoters on the same scale. </p>
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<p id="more"><a href="/Team:Uppsala_University/Promoters">Learn more</a></p></div>
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<h2>Chromoproteins</h2>
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<p id="second">Proteins with an intrinsic visible color are the simplest possible reporters i molecular biology. Most iGEM:ers are familiar with the Red Flourescent Protein (RFP), but there are many other colors aviable among all organism of the world. We have characterized and submitted new chromoproteins, allowing multiplexed colorful reporters. </p>
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<h2>Scarless gene deletion</h2>
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<p id="second">In our project we have extensivly used recombineering onto the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides  easy and reliable recombineering and the possibility of scarless gene deletion. <a href="https://2012.igem.org/Team:Uppsala_University/Catsac"> Read more...</a</p></div>
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<p>We have constructed a range of new standard backbones, especially low copy backbones and backbones with built-in lacIq repression for tight control of toxic genes. This work was induced as it turned out that the common registry pSB4 backbones all have a faulty copy number regulation, while we needed low copy backbones for out project. <a href="https://2012.igem.org/Team:Uppsala_University/Backbones"> Read more...</a></p>
 
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<p><b>Scarless gene deletion</b></p>
 
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<p>In our project we have extensivly used recombineering onto the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides very easy and reliable recombineering and the possibility of scarless gene deletion. <a href="https://2012.igem.org/Team:Uppsala_University/Catsac"> Read more...</a></p>
 
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Revision as of 16:37, 25 September 2012

Team Uppsala University – iGEM 2012



sRNA gene silencing

We have developed a modular screening system and protocol for finding silencing sRNA:s against arbitrary genes. Using this, we have found a strongly silencing sRNA:s against a clinical antibiotic gene and lowered the minimary inhibatory concentration five-fold in resistant bacteria.

Read more...

New standard backbone series

We have constructed a range of new standard backbones, especially low copy backbones and backbones with built-in lacIq repression for tight control of toxic genes. This work was induced as it turned out that the common registry pSB4 backbones all have a faulty copy number regulation, while we needed low copy backbones for out project. Read more...
 

Promoter characterization

We have characterized promoter strengths of ten popular promoters with Fluorescense Activated Cell Sorting (FACS), putting syntetic and wild-type promoters on the same scale.

Learn more

Chromoproteins

Proteins with an intrinsic visible color are the simplest possible reporters i molecular biology. Most iGEM:ers are familiar with the Red Flourescent Protein (RFP), but there are many other colors aviable among all organism of the world. We have characterized and submitted new chromoproteins, allowing multiplexed colorful reporters.

Learn more

Scarless gene deletion

In our project we have extensivly used recombineering onto the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides easy and reliable recombineering and the possibility of scarless gene deletion. Read more...


Sponsors






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