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Team:MIT/MaterialsAndMethods
From 2012.igem.org
(Difference between revisions)
| Line 76: | Line 76: | ||
<div class="bio" id="iv1bio"> | <div class="bio" id="iv1bio"> | ||
<h1> Gate Anneals </h1> | <h1> Gate Anneals </h1> | ||
| - | <h2> Room Temperature Annealing <h2> | + | <h2><b> Room Temperature Annealing </b><h2> |
| - | + | <OL> | |
<LI>Mix gate and output in a 1.2:1 ratio in a tube.</LI> | <LI>Mix gate and output in a 1.2:1 ratio in a tube.</LI> | ||
| - | <LI> | + | <LI>Cover the tube in foil, if you didn't use an opaque/black tube.</LI> |
<LI>Leave the tube on a bench top overnight.</LI> | <LI>Leave the tube on a bench top overnight.</LI> | ||
</OL> | </OL> | ||
| + | <h2><b> Room Temperature Annealing </b><h2> | ||
| + | <OL> | ||
| + | <LI>Preheat the heat block to 95C, add water to the well you will be using.</LI> | ||
| + | <LI>Put a piece of styrofoam (covered with foil) on the heat block. Make sure the temperature is still at 95C, and doesn't jump</LI> | ||
| + | <LI>Mix gate and output in a 1.2:1 ratio in a tube, put it in the heat block.</LI> | ||
| + | <LI>Cover again with the styrofoam, set the heat block to RT.</LI> | ||
| + | <LI>5. Wait until the block cools. It will probably not reach RT, but will be at around 30C after a few hours. Turn the block off completely and wait for a bit (1h).</LI> | ||
| + | </OL> | ||
Revision as of 23:14, 9 September 2012
Gate Anneals
Room Temperature Annealing
- Mix gate and output in a 1.2:1 ratio in a tube.
- Cover the tube in foil, if you didn't use an opaque/black tube.
- Leave the tube on a bench top overnight.
Room Temperature Annealing
- Preheat the heat block to 95C, add water to the well you will be using.
- Put a piece of styrofoam (covered with foil) on the heat block. Make sure the temperature is still at 95C, and doesn't jump
- Mix gate and output in a 1.2:1 ratio in a tube, put it in the heat block.
- Cover again with the styrofoam, set the heat block to RT.
- 5. Wait until the block cools. It will probably not reach RT, but will be at around 30C after a few hours. Turn the block off completely and wait for a bit (1h).
- Mix gate and output in a 1.2:1 ratio in a tube.
- Cover the tube in foil, if you didn't use an opaque/black tube.
- Leave the tube on a bench top overnight.
Room Temperature Annealing
- Preheat the heat block to 95C, add water to the well you will be using.
- Put a piece of styrofoam (covered with foil) on the heat block. Make sure the temperature is still at 95C, and doesn't jump
- Mix gate and output in a 1.2:1 ratio in a tube, put it in the heat block.
- Cover again with the styrofoam, set the heat block to RT.
- 5. Wait until the block cools. It will probably not reach RT, but will be at around 30C after a few hours. Turn the block off completely and wait for a bit (1h).
- Preheat the heat block to 95C, add water to the well you will be using.
- Put a piece of styrofoam (covered with foil) on the heat block. Make sure the temperature is still at 95C, and doesn't jump
- Mix gate and output in a 1.2:1 ratio in a tube, put it in the heat block.
- Cover again with the styrofoam, set the heat block to RT.
- 5. Wait until the block cools. It will probably not reach RT, but will be at around 30C after a few hours. Turn the block off completely and wait for a bit (1h).
