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Team:Freiburg/Project/Tal
From 2012.igem.org
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=<span style="color:#2244AA"> Step 1. The Experiment = | =<span style="color:#2244AA"> Step 1. The Experiment = | ||
| - | First you need to think about your experiment and what you want to do, when working with TAL proteins its pretty clear you want to target a sequence. | + | First you need to think about your experiment and what you want to do, when working with TAL proteins its pretty clear you want to target a dna sequence. To choose your sequence you need to know some of the limitations of TAL proteins to pick it the right way. |
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| + | <b>1. Every TAL protein starts and ends with a Thymin</b> | ||
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| + | This Thymin is already inserted in front of your TAL protein when you use our Toolbox but it should also be in front of the first base of your sequence | ||
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| + | <b>2. Your sequence must be twelve bases long</b> | ||
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| + | Our Toolbox is optimized for sequences of twelve plus two (the Thymin at upstream and downstrem) this lenght guarantes a high enough specifity and at the same time a librarysize that good to handle. | ||
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| + | If you found a sequence that suits this limitations you can start building your TAL protein. | ||
Revision as of 14:03, 9 September 2012
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| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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| Building a Toolkit | Using the Toolkit | Experiments |
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Using the Toolkit
Here we try to give you something like a short manual on how to use our toolkit to design TAL poteins.
Step 1. The Experiment
First you need to think about your experiment and what you want to do, when working with TAL proteins its pretty clear you want to target a dna sequence. To choose your sequence you need to know some of the limitations of TAL proteins to pick it the right way.
1. Every TAL protein starts and ends with a Thymin
This Thymin is already inserted in front of your TAL protein when you use our Toolbox but it should also be in front of the first base of your sequence
2. Your sequence must be twelve bases long
Our Toolbox is optimized for sequences of twelve plus two (the Thymin at upstream and downstrem) this lenght guarantes a high enough specifity and at the same time a librarysize that good to handle.
If you found a sequence that suits this limitations you can start building your TAL protein.
