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Team:TU Darmstadt/Labjournal/Metabolism
From 2012.igem.org
(Difference between revisions)
(→week 1 (14.-18.05.12)) |
(→week 2 (21.-25.05.12)) |
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| Line 17: | Line 17: | ||
===week 2 (21.-25.05.12)=== | ===week 2 (21.-25.05.12)=== | ||
'''tphA1''' | '''tphA1''' | ||
| + | * Isolation of the gene from ''C. testosteroni KF-1'' genome using [[colony-PCR]] | ||
| + | ** Annealing temperature: 49 °C | ||
| + | ** [[Primer]]: tphA1-l-F and tphA1-l-R | ||
'''tphA2''' | '''tphA2''' | ||
| - | + | * No work progress | |
'''tphA3''' | '''tphA3''' | ||
| - | + | * Isolation of the gene from ''C. testosteroni KF-1'' genome using [[colony-PCR]] | |
| + | ** Annealing temperature: 59 °C | ||
| + | ** [[Primer]]: tphA3-l-F and tphA3-l-R | ||
'''tphB''' | '''tphB''' | ||
| - | + | * Isolation of the gene from ''C. testosteroni KF-1'' genome using [[colony-PCR]] | |
| + | ** Annealing temperature: 59 °C | ||
| + | ** [[Primer]]: tphB-l-F and tphB-l-R | ||
'''aroY''' | '''aroY''' | ||
| - | + | * No work progress | |
'''Other''' | '''Other''' | ||
| + | * [[Transformation]] and [[midi prep]] of all used [[Biobricks]] | ||
| + | ** Concentraions measured by [[Nanoprop]] | ||
| + | ::{| class="wikitable" | ||
| + | |- | ||
| + | ! Biobrick!! Concentraion [ng/µl] | ||
| + | |- | ||
| + | | BBa_K316003 || - | ||
| + | |- | ||
| + | | BBa_J23100 || - | ||
| + | |- | ||
| + | | BBa_B0015 || - | ||
| + | |- | ||
| + | | BBa_J61101 || - | ||
| + | |} | ||
===week 3 (28.05.-01.06.12)=== | ===week 3 (28.05.-01.06.12)=== | ||
Revision as of 08:56, 9 September 2012
week 1 (14.-18.05.12)
tphA1
- No work progress
tphA2
- No work progress
tphA3
- No work progress
tphB
- No work progress
aroY
- No work progress
Other
- Reconstitution of C. testosteroni KF-1 according to DSMZ [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf protocol]
- Cultivation of C. testosteroni KF-1 on agar plates with Medium 1
- Production of chemically competent E. coli DH5α and E. coli BL21(DE3)pLysS cells
week 2 (21.-25.05.12)
tphA1
- Isolation of the gene from C. testosteroni KF-1 genome using colony-PCR
- Annealing temperature: 49 °C
- Primer: tphA1-l-F and tphA1-l-R
tphA2
- No work progress
tphA3
- Isolation of the gene from C. testosteroni KF-1 genome using colony-PCR
- Annealing temperature: 59 °C
- Primer: tphA3-l-F and tphA3-l-R
tphB
- Isolation of the gene from C. testosteroni KF-1 genome using colony-PCR
- Annealing temperature: 59 °C
- Primer: tphB-l-F and tphB-l-R
aroY
- No work progress
Other
- Transformation and midi prep of all used Biobricks
- Concentraions measured by Nanoprop
Biobrick Concentraion [ng/µl] BBa_K316003 - BBa_J23100 - BBa_B0015 - BBa_J61101 -
week 3 (28.05.-01.06.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 4 (04.-08.06.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 5 (11.-15-06.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 6 (18.-22.06.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 7 (25.-29.06.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 8 (02.-06.07.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 9 (09.-13.07.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 10 (16.-20.07.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 11 (23.-27.07.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 12 (30.07.-03.08.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 13 (06.-10.08.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 14 (13.-17.08.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 15 (20.-24.08.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 16 (27.-31.08.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 17 (03.-07.09.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 18 (10.-17.09.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
week 19 (17.-21.09.12)
tphA1
tphA2
tphA3
tphB
aroY
Other
