Team:KIT-Kyoto/c6h12o6

From 2012.igem.org

(Difference between revisions)

Revision as of 00:31, 9 September 2012

1 August

1-1	sample1
2-1	6μL
3-1	10μL
4-1	10μL
5-1	3.2μL
6-1	3μL
7-1	3μL
8-1	2μL
9-1	62.8μL
10-1	100μL

temperature	time	cycle
95°C	2min
95°C	15sec	25cycle
60°C	30sec	25cycle
68°C	2min30sec	25cycle
68°C	2min30sec
14°C	∞

1-1	sample1	sample2	sample3	sample4
2-1	2μL	2μL	1μL	1μL
3-1	2μL	2μL	2μL	2μL
4-1	2μL	2μL	2μL	2μL
5-1	0.8μL	0.8μL	0.8μL	0.8μL
6-1	0.6μL	0.6μL	0.6μL	0.6μL
7-1	0.6μL	0.6μL	0.6μL	0.6μL
8-1	0.4μL	0.4μL	0.4μL	0.4μL
9-1	11.6μL	11.6μL	12.6μL	12.6μL
10-1	20μL	20μL	20μL	20μL

temperature	time	cycle
95°C	2min
95°C	15sec	25cycle
50°C(sample1,3)or55°C(sample2,4)	30sec	25cycle
68°C	3min30sec	25cycle
68°C	3min30sec
14°C	∞

2 August

1-1	1-2	1-3
2-1	10μL	20μL
3-1	2μL	4μL
4-1	12μL	25μL

	1-2
DNA sample	90μL
6×Dye	18μL
4-1	108μL

1-1	5μL
2-1	10μL
3-1	10μL
4-1	4μL
5-1	3μL
6-1	3μL
7-1	2μL
8-1	63μL
9-1	100μL

temperature	time	cycle
95°C	2min
95°C	15sec	35cycle
55°C	30sec	35cycle
68°C	3min30sec	35cycle
68°C	3min30sec
14°C	∞

Result

3 August

1-1	1μL
2-1	0.35μL
3-1	7.65
4-1	9μL

1-1	sample1	sample2
2-1	2.5μL	2.5μL
3-1	5μL	5μL
4-1	5μL	5μL
5-1	2μL	2.4μL
6-1	1.5μL	1.5μL
7-1	1.5μL	1.5μL
8-1	1μL	1μL
9-1	31.5μL	31.1μL
10-1	50μL	50μL

temperature	time	cycle
95°C	2min
95°C	15sec	35cycle
55°C	30sec	35cycle
68°C	3min30sec	35cycle
68°C	3min30sec
14°C	∞

4 August

5 August

6 August

7 August

pDONR

		BP TNFA-1	BP TNFA-2
DNA sample	1μL	1μL	1μL
3-1	1μL	1μL	1μL
4-1	4μL	4μL	4μL
5-1	6μL	6μL	6μL

1-1	2μL
2-1	0.5μL
3-1	6.5μL
4-1	9μL

8 August

9 Augus

1-1	1μL
2-1	0.5μL
3-1	0.2μL
4-1	3.3μL
5-1	5μL

1-1	1-2
2-1	1μL
3-1	2μL
4-1	2μL
5-1	0.8μL
6-1	0.6μL
7-1	0.6μL
8-1	0.4μL
9-1	12.6μL
10-1	20μL

temperature	time	cycle
95°C	2min
95°C	15sec	25cycle
55°C	30sec	25cycle
68°C	2min30sec	25cycle
68°C	2min30sec
14°C	∞

10 August

11 August

12 August

13 August

1-1	1μL
2-1	0.5μL
3-1	0.2μL
4-1	3.3μL
5-1	5μL

14 August

1-1	sample1
2-1	6μL
3-1	10μL
4-1	10μL
5-1	3.2μL
6-1	3μL
7-1	3μL
8-1	2μL
9-1	62.8μL
10-1	100μL

temperature	time	cycle
95°C	2min
95°C	15sec	25cycle
60°C	30sec	25cycle
68°C	2min30sec	25cycle
68°C	2min30sec
14°C	∞

15 August

16 August

17 August

18 August

19 August

20 August

1-1	1-2
2-1	2-2
3-1	3-2
4-1	4-2

21 August

1-1	1-2
2-1	2-2
3-1	3-2
4-1	4-2

@@ Line 85: / Line 85: @@
 <Table Border Cellspacing="0">
 <Tr><Td>1-1</Td><Td>1-2</Td><Td>1-3</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td><Td>2-3</Td></Tr>
+<Tr><Td>2-1</Td><Td>10μL</Td><Td>20μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td><Td>3-3</Td></Tr>
+<Tr><Td>3-1</Td><Td>2μL</Td><Td>4μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td><Td>4-3</Td></Tr>
+<Tr><Td>4-1</Td><Td>12μL</Td><Td>25μL</Td></Tr>
 </Table>
 <br>
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td></Tr>
+<Tr><Td></Td><Td>1-2</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td></Tr>
+<Tr><Td>DNA sample</Td><Td>90μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td></Tr>
+<Tr><Td>6×Dye</Td><Td>18μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td></Tr>
+<Tr><Td>4-1</Td><Td>108μL</Td></Tr>
 </Table>
 <br>
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td></Tr>
+<Tr><Td>1-1</Td><Td>5μL</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td></Tr>
+<Tr><Td>2-1</Td><Td>10μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td></Tr>
+<Tr><Td>3-1</Td><Td>10μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td></Tr>
+<Tr><Td>4-1</Td><Td>4μL</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td></Tr>
+<Tr><Td>5-1</Td><Td>3μL</Td></Tr>
-<Tr><Td>6-1</Td><Td>6-2</Td></Tr>
+<Tr><Td>6-1</Td><Td>3μL</Td></Tr>
-<Tr><Td>7-1</Td><Td>7-2</Td></Tr>
+<Tr><Td>7-1</Td><Td>2μL</Td></Tr>
-<Tr><Td>8-1</Td><Td>8-2</Td></Tr>
+<Tr><Td>8-1</Td><Td>63μL</Td></Tr>
-<Tr><Td>9-1</Td><Td>9-2</Td></Tr>
+<Tr><Td>9-1</Td><Td>100μL</Td></Tr>
 </Table>
 <br>
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td><Td>1-3</Td></Tr>
+<Tr><Td>temperature</Td><Td>time</Td><Td>cycle</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td><Td>2-3</Td></Tr>
+<Tr><Td>95°C</Td><Td>2min</Td><Td></Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td><Td>3-3</Td></Tr>
+<Tr><Td>95°C</Td><Td>15sec</Td><Td>35cycle</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td><Td>4-3</Td></Tr>
+<Tr><Td>55°C</Td><Td>30sec</Td><Td>35cycle</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td><Td>5-3</Td></Tr>
+<Tr><Td>68°C</Td><Td>3min30sec</Td><Td>35cycle</Td></Tr>
-<Tr><Td>6-1</Td><Td>6-2</Td><Td>6-3</Td></Tr>
+<Tr><Td>68°C</Td><Td>3min30sec</Td><Td></Td></Tr>
-<Tr><Td>7-1</Td><Td>7-2</Td><Td>7-3</Td></Tr>
+<Tr><Td>14°C</Td><Td>∞</Td><Td></Td></Tr>
 </Table>
 <br>
@@ Line 145: / Line 145: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td></Tr>
+<Tr><Td>1-1</Td><Td>1μL</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td></Tr>
+<Tr><Td>2-1</Td><Td>0.35μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td></Tr>
+<Tr><Td>3-1</Td><Td>7.65</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td></Tr>
+<Tr><Td>4-1</Td><Td>9μL</Td></Tr>
 </Table>
 <br>
@@ Line 154: / Line 154: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td><Td>1-3</Td></Tr>
+<Tr><Td>1-1</Td><Td>sample1</Td><Td>sample2</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td><Td>2-3</Td></Tr>
+<Tr><Td>2-1</Td><Td>2.5μL</Td><Td>2.5μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td><Td>3-3</Td></Tr>
+<Tr><Td>3-1</Td><Td>5μL</Td><Td>5μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td><Td>4-3</Td></Tr>
+<Tr><Td>4-1</Td><Td>5μL</Td><Td>5μL</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td><Td>5-3</Td></Tr>
+<Tr><Td>5-1</Td><Td>2μL</Td><Td>2.4μL</Td></Tr>
-<Tr><Td>6-1</Td><Td>6-2</Td><Td>6-3</Td></Tr>
+<Tr><Td>6-1</Td><Td>1.5μL</Td><Td>1.5μL</Td></Tr>
-<Tr><Td>7-1</Td><Td>7-2</Td><Td>7-3</Td></Tr>
+<Tr><Td>7-1</Td><Td>1.5μL</Td><Td>1.5μL</Td></Tr>
-<Tr><Td>8-1</Td><Td>8-2</Td><Td>8-3</Td></Tr>
+<Tr><Td>8-1</Td><Td>1μL</Td><Td>1μL</Td></Tr>
-<Tr><Td>9-1</Td><Td>9-2</Td><Td>9-3</Td></Tr>
+<Tr><Td>9-1</Td><Td>31.5μL</Td><Td>31.1μL</Td></Tr>
-<Tr><Td>10-1</Td><Td>10-2</Td><Td>10-3</Td></Tr>
+<Tr><Td>10-1</Td><Td>50μL</Td><Td>50μL</Td></Tr>
 </Table>
 <br>
@@ Line 169: / Line 169: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td><Td>1-3</Td></Tr>
+<Tr><Td>temperature</Td><Td>time</Td><Td>cycle</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td><Td>2-3</Td></Tr>
+<Tr><Td>95°C</Td><Td>2min</Td><Td></Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td><Td>3-3</Td></Tr>
+<Tr><Td>95°C</Td><Td>15sec</Td><Td>35cycle</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td><Td>4-3</Td></Tr>
+<Tr><Td>55°C</Td><Td>30sec</Td><Td>35cycle</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td><Td>5-3</Td></Tr>
+<Tr><Td>68°C</Td><Td>3min30sec</Td><Td>35cycle</Td></Tr>
-<Tr><Td>6-1</Td><Td>6-2</Td><Td>6-3</Td></Tr>
+<Tr><Td>68°C</Td><Td>3min30sec</Td><Td></Td></Tr>
-<Tr><Td>7-1</Td><Td>7-2</Td><Td>7-3</Td></Tr>
+<Tr><Td>14°C</Td><Td>∞</Td><Td></Td></Tr>
 </Table>
 <br>
@@ Line 250: / Line 250: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td><Td>1-3</Td><Td>1-4</Td></Tr>
+<Tr><Td></Td><Td></Td>pDONR<Td>BP TNFA-1</Td><Td>BP TNFA-2</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td><Td>2-3</Td><Td>2-4</Td></Tr>
+<Tr><Td>DNA sample</Td><Td>1μL</Td><Td>1μL</Td><Td>1μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td><Td>3-3</Td><Td>3-4</Td></Tr>
+<Tr><Td>3-1</Td><Td>1μL</Td><Td>1μL</Td><Td>1μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td><Td>4-3</Td><Td>4-4</Td></Tr>
+<Tr><Td>4-1</Td><Td>4μL</Td><Td>4μL</Td><Td>4μL</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td><Td>5-3</Td><Td>5-4</Td></Tr>
+<Tr><Td>5-1</Td><Td>6μL</Td><Td>6μL</Td><Td>6μL</Td></Tr>
 </Table>
 <br>
@@ Line 260: / Line 260: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td></Tr>
+<Tr><Td>1-1</Td><Td>2μL</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td></Tr>
+<Tr><Td>2-1</Td><Td>0.5μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td></Tr>
+<Tr><Td>3-1</Td><Td>6.5μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td></Tr>
+<Tr><Td>4-1</Td><Td>9μL</Td></Tr>
 </Table>
 <br>
@@ Line 303: / Line 303: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td></Tr>
+<Tr><Td>1-1</Td><Td>1μL</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td></Tr>
+<Tr><Td>2-1</Td><Td>0.5μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td></Tr>
+<Tr><Td>3-1</Td><Td>0.2μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td></Tr>
+<Tr><Td>4-1</Td><Td>3.3μL</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td></Tr>
+<Tr><Td>5-1</Td><Td>5μL</Td></Tr>
 </Table>
 <br>
@@ Line 314: / Line 314: @@
 <Table Border Cellspacing="0">
 <Tr><Td>1-1</Td><Td>1-2</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td></Tr>
+<Tr><Td>2-1</Td><Td>1μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td></Tr>
+<Tr><Td>3-1</Td><Td>2μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td></Tr>
+<Tr><Td>4-1</Td><Td>2μL</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td></Tr>
+<Tr><Td>5-1</Td><Td>0.8μL</Td></Tr>
-<Tr><Td>6-1</Td><Td>6-2</Td></Tr>
+<Tr><Td>6-1</Td><Td>0.6μL</Td></Tr>
-<Tr><Td>7-1</Td><Td>7-2</Td></Tr>
+<Tr><Td>7-1</Td><Td>0.6μL</Td></Tr>
-<Tr><Td>8-1</Td><Td>8-2</Td></Tr>
+<Tr><Td>8-1</Td><Td>0.4μL</Td></Tr>
-<Tr><Td>9-1</Td><Td>9-2</Td></Tr>
+<Tr><Td>9-1</Td><Td>12.6μL</Td></Tr>
-<Tr><Td>10-1</Td><Td>10-2</Td></Tr>
+<Tr><Td>10-1</Td><Td>20μL</Td></Tr>
 </Table>
 <br>
@@ Line 328: / Line 328: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td><Td>1-3</Td></Tr>
+<Tr><Td>temperature</Td><Td>time</Td><Td>cycle</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td><Td>2-3</Td></Tr>
+<Tr><Td>95°C</Td><Td>2min</Td><Td></Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td><Td>3-3</Td></Tr>
+<Tr><Td>95°C</Td><Td>15sec</Td><Td>25cycle</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td><Td>4-3</Td></Tr>
+<Tr><Td>55°C</Td><Td>30sec</Td><Td>25cycle</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td><Td>5-3</Td></Tr>
+<Tr><Td>68°C</Td><Td>2min30sec</Td><Td>25cycle</Td></Tr>
-<Tr><Td>6-1</Td><Td>6-2</Td><Td>6-3</Td></Tr>
+<Tr><Td>68°C</Td><Td>2min30sec</Td><Td></Td></Tr>
-<Tr><Td>7-1</Td><Td>7-2</Td><Td>7-3</Td></Tr>
+<Tr><Td>14°C</Td><Td>∞</Td><Td></Td></Tr>
 </Table>
 <br>
@@ Line 410: / Line 410: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td></Tr>
+<Tr><Td>1-1</Td><Td>1μL</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td></Tr>
+<Tr><Td>2-1</Td><Td>0.5μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td></Tr>
+<Tr><Td>3-1</Td><Td>0.2μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td></Tr>
+<Tr><Td>4-1</Td><Td>3.3μL</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td></Tr>
+<Tr><Td>5-1</Td><Td>5μL</Td></Tr>
 </Table>
 <br>
@@ Line 437: / Line 437: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td></Tr>
+<Tr><Td>1-1</Td><Td>sample1</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td></Tr>
+<Tr><Td>2-1</Td><Td>6μL</Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td></Tr>
+<Tr><Td>3-1</Td><Td>10μL</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td></Tr>
+<Tr><Td>4-1</Td><Td>10μL</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td></Tr>
+<Tr><Td>5-1</Td><Td>3.2μL</Td></Tr>
-<Tr><Td>6-1</Td><Td>6-2</Td></Tr>
+<Tr><Td>6-1</Td><Td>3μL</Td></Tr>
-<Tr><Td>7-1</Td><Td>7-2</Td></Tr>
+<Tr><Td>7-1</Td><Td>3μL</Td></Tr>
-<Tr><Td>8-1</Td><Td>8-2</Td></Tr>
+<Tr><Td>8-1</Td><Td>2μL</Td></Tr>
-<Tr><Td>9-1</Td><Td>9-2</Td></Tr>
+<Tr><Td>9-1</Td><Td>62.8μL</Td></Tr>
-<Tr><Td>10-1</Td><Td>10-2</Td></Tr>
+<Tr><Td>10-1</Td><Td>100μL</Td></Tr>
 </Table>
 <br>
@@ Line 452: / Line 452: @@
 <Table Border Cellspacing="0">
-<Tr><Td>1-1</Td><Td>1-2</Td><Td>1-3</Td></Tr>
+<Tr><Td>temperature</Td><Td>time</Td><Td>cycle</Td></Tr>
-<Tr><Td>2-1</Td><Td>2-2</Td><Td>2-3</Td></Tr>
+<Tr><Td>95°C</Td><Td>2min</Td><Td></Td></Tr>
-<Tr><Td>3-1</Td><Td>3-2</Td><Td>3-3</Td></Tr>
+<Tr><Td>95°C</Td><Td>15sec</Td><Td>25cycle</Td></Tr>
-<Tr><Td>4-1</Td><Td>4-2</Td><Td>4-3</Td></Tr>
+<Tr><Td>60°C</Td><Td>30sec</Td><Td>25cycle</Td></Tr>
-<Tr><Td>5-1</Td><Td>5-2</Td><Td>5-3</Td></Tr>
+<Tr><Td>68°C</Td><Td>2min30sec</Td><Td>25cycle</Td></Tr>
-<Tr><Td>6-1</Td><Td>6-2</Td><Td>6-3</Td></Tr>
+<Tr><Td>68°C</Td><Td>2min30sec</Td><Td></Td></Tr>
-<Tr><Td>7-1</Td><Td>7-2</Td><Td>7-3</Td></Tr>
+<Tr><Td>14°C</Td><Td>∞</Td><Td></Td></Tr>
 </Table>
 <br>

Team:KIT-Kyoto/c6h12o6

From 2012.igem.org

Revision as of 00:31, 9 September 2012

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