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From 2012.igem.org
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-- The mixture is kept for 20 minutes at 80ºC | -- The mixture is kept for 20 minutes at 80ºC | ||
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After those 20 minutes, the plasmid pSB1C3 is ready to use. | After those 20 minutes, the plasmid pSB1C3 is ready to use. | ||
Revision as of 13:46, 7 September 2012
Digestion protocol
- 8 uL DNA linearized plasmid Backbone (25 ng/uL)
- 1uL PstI
- 1uL EcoRI
- 2.5 uL H Buffer
- X uL Water
TOTAL 25 uL
-- The digestion mixture is kept for 3 hours at 37 ° C
-- The mixture is kept for 20 minutes at 80ºC
After those 20 minutes, the plasmid pSB1C3 is ready to use.
