Team:Macquarie Australia/Protocols/NanoDropResults

From 2012.igem.org

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<center><h1>NanoDrop Results</h1></center>
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<br/>
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'''Table 1''' - NanoDrop results of purified plasmids, prepared by Gibson Assembly, including DNA concentration (ng/uL), and 260/280 and 260/230 absorbance values.
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{| border="3" cellpadding="4" cellspacing="0" align="center" style="width: 55%; height: 400px"
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|-
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! scope="col"| Date
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! scope="col"| Plasmid
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! scope="col"| Colony #
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! scope="col"| Concentration (ng/uL)
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! scope="col"| 260/280
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! scope="col"| 260/230
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|-
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| align="center" rowspan="2" | 6 Aug
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| align="center" rowspan="2" | 3K
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| align="center"| 1
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| align="center"| 50.70
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| align="center"| 1.89
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| align="center"| 2.32
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|-
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| align="center"| 2
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| align="center"| 61.40
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| align="center"| 1.88
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| align="center"| 1.97
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|-
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| align="center" rowspan="7" | 7 Aug
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| align="center" rowspan="6" | 1C
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| align="center"| 1
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| align="center"| 61.90
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| align="center"| 1.90
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| align="center"| 2.30
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|-
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| align="center"| 2
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| align="center"| 52.10
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| align="center"| 1.90
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| align="center"| 2.42
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|-
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| align="center"| 3
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| align="center"| 52.00
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| align="center"| 1.91
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| align="center"| 2.11
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|-
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| align="center"| 4
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| align="center"| 69.00
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| align="center"| 1.88
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| align="center"| 2.15
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|-
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| align="center"| 5
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| align="center"| 65.60
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| align="center"| 1.89
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| align="center"| 2.38
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|-
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| align="center"| 6
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| align="center"| 58.70
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| align="center"| 1.88
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| align="center"| 2.40
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|-
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| align="center"| 3K
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| align="center"| 1
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| align="center"| 6.80
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| align="center"| 2.16
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| align="center"| 0.56
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|-
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| align="center" rowspan="2" | 11 Aug
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| align="center" rowspan="2" | 2K
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| align="center"| 1
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| align="center"| 10.90
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| align="center"| 1.98
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| align="center"| 2.68
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|-
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| align="center"| 2
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| align="center"| 17.50
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| align="center"| 1.90
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| align="center"| 2.48
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|-
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| align="center" rowspan="9" | 13 Aug
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| align="center" rowspan="3" | 3CE
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| align="center"| 1
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| align="center"| 43.60
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| align="center"| 1.92
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| align="center"| 2.27
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|-
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| align="center"| 2
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| align="center"| 38.30
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| align="center"| 1.89
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| align="center"| 1.67
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|-
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| align="center"| 3
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| align="center"| 62.20
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| align="center"| 1.91
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| align="center"| 1.77
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|-
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| align="center" rowspan="3" | 4KE
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| align="center"| 1
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| align="center"| 49.50
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| align="center"| 1.82
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| align="center"| 1.23
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|-
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| align="center"| 2
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| align="center"| 32.30
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| align="center"| 1.78
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| align="center"| 0.91
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|-
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| align="center"| 3
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| align="center"| 14.40
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| align="center"| 1.89
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| align="center"| 1.75
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|-
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| align="center" rowspan="3" | 5CE
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| align="center"| 1
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| align="center"| 71.10
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| align="center"| 1.76
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| align="center"| 1.17
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|-
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| align="center"| 2
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| align="center"| 92.10
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| align="center"| 1.78
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| align="center"| 1.17
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|-
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| align="center"| 3
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| align="center"| 56.70
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| align="center"| 1.86
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| align="center"| 2.08
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|}
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<br/>

Latest revision as of 01:23, 23 September 2012



NanoDrop Results


Table 1 - NanoDrop results of purified plasmids, prepared by Gibson Assembly, including DNA concentration (ng/uL), and 260/280 and 260/230 absorbance values.

Date Plasmid Colony # Concentration (ng/uL) 260/280 260/230
6 Aug 3K 1 50.70 1.89 2.32
2 61.40 1.88 1.97
7 Aug 1C 1 61.90 1.90 2.30
2 52.10 1.90 2.42
3 52.00 1.91 2.11
4 69.00 1.88 2.15
5 65.60 1.89 2.38
6 58.70 1.88 2.40
3K 1 6.80 2.16 0.56
11 Aug 2K 1 10.90 1.98 2.68
2 17.50 1.90 2.48
13 Aug 3CE 1 43.60 1.92 2.27
2 38.30 1.89 1.67
3 62.20 1.91 1.77
4KE 1 49.50 1.82 1.23
2 32.30 1.78 0.91
3 14.40 1.89 1.75
5CE 1 71.10 1.76 1.17
2 92.10 1.78 1.17
3 56.70 1.86 2.08


Retrieved from "http://2012.igem.org/Team:Macquarie_Australia/Protocols/NanoDropResults"