Team:TU Darmstadt/Protocols

From 2012.igem.org

(Difference between revisions)

Latest revision as of 21:45, 26 September 2012

Protocols

The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.

In vivo

[http://en.wikipedia.org/wiki/E._coli Escherichia coli]

Comamonas testosteroni

[http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]

In vitro

Nucleic acids

[http://en.wikipedia.org/wiki/DNA DNA]

Agarose gel electrophoresis
Ammonium acetate precipitation
Cloning
Dephosphorylation
DNA Ligation
DNA Ligation à la group transport
DNA Quantification / NanoDrop
Miniprep
Mutagenic PCR
PCR
Purification of DNA
Restriction digest
Salting Out
[http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA]

@@ Line 23: / Line 23: @@
 		    <li><a href="/Team:TU_Darmstadt/Labjournal" title="Labjournal">Labjournal</a></li>
 		    <li><a href="/Team:TU_Darmstadt/Protocols" title="Protocols">Protocols</a></li>
+			<li><a href="/Team:TU_Darmstadt/Materials" title="Materials">Materials</a></li>
+			<li><a href="/Team:TU_Darmstadt/Modeling" title="Modeling">Modeling</a></li>
 		    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
 		    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
-		<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a><ul>
+		<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li>
-		    <li><a href="/Team:TU_Darmstadt/Human_Practice/Panel_Discussion " title="Panel Discussion">Panel Discussion</a></li>
-		    <li><a href="/Team:TU_Darmstadt/Human_Practice/Symposia" title="Symposia">Symposia</a></li>
-		    <li><a href="/Team:TU_Darmstadt/Human_Practice/Classes" title="Classes">Classes</a></li></ul></li>
 		<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
 		    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
 		    <li><a href="/Team:TU_Darmstadt/Contact" title="Contact">Benefits</a></li></ul></li>
 	</ul>
+<div id="igem"><a href="https://2012.igem.org/Main_Page"></a></div>
 </div>
 <!-- end #menu -->
 </html>
 <span style="font-size:200%;"><span style="color:#00689D;">Protocols</span></span>
-== Protocols ==
-The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012.
+The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the [https://2012.igem.org iGEM] PET.erminators [https://2012.igem.org/Team:TU_Darmstadt/Project project 2012]. The corresponding [https://2012.igem.org/Team:TU_Darmstadt/Materials materials] can be found in the [https://2012.igem.org/Team:TU_Darmstadt/Materials materials section].
 == In vivo ==
@@ Line 49: / Line 50: @@
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR Engineering BioBrick vectors from BioBrick parts/Colony PCR]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Glycerine_stock Glycerine Stocks]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/In-fusion_BioBrick_assembly In-fusion BioBrick assembly]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heat Shock Transformation]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep_/_Qiagen_kit Miniprep / Qiagen kit]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Fermentas_kit Miniprep / Fermentas kit]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Transformation_of_chemically_competent_cells Transformation of chemically competent cells]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Promega_kit Miniprep / Promega kit]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Qiagen_kit Miniprep / Qiagen kit]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_Est13 Protein Expression Est13]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_FsC Protein Expression FsC]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot]
 === [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] ===
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Reviving_a_freez_dried_cell_culture Reviving a freez dried cell culture]
+* [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
-*
 == In vitro ==
@@ Line 63: / Line 66: @@
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SOE-PCR SOE-PCR]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR#SOE_PCR SOE-PCR]
 === [http://en.wikipedia.org/wiki/DNA DNA] ===
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrohporesis Agarose gel electrohporesis]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Annealing_and_primer_extension Annealing and primer extension]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Ammonium_sulfate_precipitation Ammonium acetate precipitation]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Annealing_primers Annealing primers]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Assembly_PCR Assembly PCR]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ DNA Ligation à la group transport]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Synthesis_from_Oligos DNA Synthesis from Oligos]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR Engineering BioBrick vectors from BioBrick parts/Colony PCR]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep]
-* [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Polyacrylamide_gel_electrophoresis Polyacrylamide gel electrophoresis]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Primer_Design Primer Design]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA  Purification of DNA]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Resuspension_of_primers Resuspension of primers]
 * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Sequencing_DNA Sequencing DNA]
+* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA]
-=== [http://en.wikipedia.org/wiki/RNA RNA] ===
-=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] ===
-== In silico ==
+=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] ===
-=== Data analysis ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Activity_assay Activity assay]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/R_Programming R Programming]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Affinity_chromatography Affinity chromatography]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Mathlab_/_Simulink Mathlab / Simulink]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/IMAC Immobilized metal ion affinity chromatography (IMAC)]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Bacteria pNP-Assay Bacteria]
-=== Databases ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Enzyme pNP-Assay Enzyme]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PDB PDB]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Proteinpurification Proteinpurification]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/CAS CAS]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page]
-=== Modelling ===
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Modelling]
-=== Sequence analysis ===
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/BLAST BLAST]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SAGE SAGE]
-=== Structure analysis ===
+== Material Science ==
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PDB PDB]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_paranitrophenylesters_with_acyl_chlorides_in_presence_of_triethylamine Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_structure_prediction Protein structure prediction]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_polyethylene_terephthalate_in_presence_of_sulfuric_acid Synthesis of polyethylene terephthalate in presence of sulfuric acid]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/RNA_secondary_structure_prediction RNA secondary structure prediction]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Surface_analysis_of_polyethylene_terephthalate_with_atomic_force_microscopy Surface analysis of polyethylene terephthalate with atomic force microscopy]
 == Miscellaneous ==
-=== Cell culture ===
+=== Staining ===
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Pouring_LB_plates Pouring LB plates]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Antibody_staining Antibody staining]
-=== Microscopy ===
+=== Analytics ===
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Determining_an_objects_size Determining an objects size]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/TEM-EDX TEM-EDX Measurements]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Preparing_slides Preparing slides]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/GC-MS GC-MS Measurements]
-* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Staining Staining]
+* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SamplePreparationEDX Preparation of Escherichia Coli for the energy dispersive X-ray spectroscopy (EDX)]
 === ILDs / Improvised Lab Devices ===

Team:TU Darmstadt/Protocols

From 2012.igem.org

Latest revision as of 21:45, 26 September 2012

Contents

In vivo

[http://en.wikipedia.org/wiki/E._coli Escherichia coli]

Comamonas testosteroni

In vitro

Nucleic acids

[http://en.wikipedia.org/wiki/DNA DNA]

Protein

Material Science

Miscellaneous

Staining

Analytics

ILDs / Improvised Lab Devices