From 2012.igem.org
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| - | Lux-Tet hybrid promoter assay </div>
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| - | __NOTOC__
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| - | =Abstract=
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| - | We succeeded in constructing and characterizing a NEW Biobrick device (name). This device is a LasR generator We found that the device is (explain)
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| - | =Introduction=
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| - | TEXT
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| - | =Rusult=
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| - | Text
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| - | =Conclusion=
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| - | Text
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| - | =Discussion=
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| - | Text
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| - | =Material&Method=
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| - | ==Construction==
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| - | ==Samples==
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| - | ==Strain==
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| - | ==Protocol==
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| - | 1.Prepare overnight culture at 37 degrees for 12hours. (2 tubes for each sample)
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| - | 2.Take 30 μl of the overnight culture of reporter cell into LB + antibiotics (Amp + Kan). (→fresh culture)
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| - | 3.Incubate the flesh culture of reporter cell] until the observed O.D. reaches around 0.60. and gather the supernatant of culture of inducer cell.
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| - | 4.Each sample of reporter cell was divided into 2. Prepare and add AHL mixture to one, cell-synthesized AHL to another one, and DMSO mixture to the other.
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| - | 5.Induction of reporter cell for 3 hours.
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| - | 6.Fluorometer (FLA5200) and flow cytometry measurements for GFP expression of reporter cell
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Latest revision as of 00:04, 27 October 2012
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