Team:Tokyo Tech/Projects/3OC6HSL→pLuxLasI-PLas/index.htm

From 2012.igem.org

(Difference between revisions)
(Created page with "{{tokyotechcss}} <div class="whitebox"> {{tokyotechtop}} </div> {{tokyotechmenubar}} <div class="whitebox"> <div id="tokyotech" style=" font:bold ;left ; font-size: 50px; color: ...")
(→Protocol)
 
(8 intermediate revisions not shown)
Line 1: Line 1:
{{tokyotechcss}}
{{tokyotechcss}}
-
<div class="whitebox">
 
{{tokyotechtop}}
{{tokyotechtop}}
-
</div>
 
{{tokyotechmenubar}}
{{tokyotechmenubar}}
<div class="whitebox">
<div class="whitebox">
-
<div id="tokyotech" style=" font:bold ;left ; font-size: 50px; color: #1E90FF; padding: 10px;">
+
<div id="tokyotech" style=" font:bold ;left ; font-size: 30px; color: #1E90FF; padding: 10px;">
-
LasR generator </div>
+
3OC6HSL dependent 3OC12HSL producer </div>
</div class="whitebox">
</div class="whitebox">
<div class="whitebox">
<div class="whitebox">
Line 19: Line 17:
Text
Text
=Conclusion=
=Conclusion=
-
Text
 
-
=Discussion=
 
Text
Text
=Material&Method=
=Material&Method=
Line 27: Line 23:
==Strain==
==Strain==
==Protocol==
==Protocol==
-
1.Prepare overnight culture at 37 degrees for 12hours. (2 tubes for each sample)
+
Text
-
 
+
-
2.Take 30 μl of the overnight culture of reporter cell into LB + antibiotics (Amp + Kan). (→fresh culture)
+
-
 
+
-
3.Incubate the flesh culture of reporter cell] until the observed O.D. reaches around 0.60. and gather the supernatant of culture of inducer cell.
+
-
 
+
-
4.Each sample of reporter cell was divided into 2. Prepare and add AHL mixture to one, cell-synthesized AHL to another one, and DMSO mixture to the other.
+
-
 
+
-
5.Induction of reporter cell for 3 hours.
+
-
 
+
-
6.Fluorometer (FLA5200) and flow cytometry measurements for GFP expression of reporter cell
+

Latest revision as of 03:18, 22 September 2012

bar

Tokyotechlogo2012.png

3OC6HSL dependent 3OC12HSL producer

Abstract

We succeeded in constructing and characterizing a NEW Biobrick device (name). This device is a LasR generator We found that the device is (explain)

Introduction

TEXT

Rusult

Text

Conclusion

Text

Material&Method

Construction

Samples

Strain

Protocol

Text
Retrieved from "http://2012.igem.org/Team:Tokyo_Tech/Projects/3OC6HSL%E2%86%92pLuxLasI-PLas/index.htm"