Team:Goettingen

From 2012.igem.org

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{{GoettingenHeader|deu=Team:Goettingen/main_deu|eng=Team:Goettingen}}
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__TOC__
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                <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
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== Welcome to iGEM G&ouml;ttingen ==
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                <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&amp;action=creativecommons" rel="meta">
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In November 2011, most of us heard about iGEM competition for the first time.
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<link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=rss">
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Erik, inspired by his former colleagues' enthusiastic reports, was looking for like-minded people at his new university that
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<link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=atom">
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were willing to organize a whole project in the field of Synthetic Biology. He described the idea of the contest to anybody willing to listen. It did not take
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long to find five other interested students. Thus, the so called organization task force of the first iGEM team of the University of Göttingen was formed.
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Luckily, the iGEM competition was already well known among the University´s professors and the team found their first supporters: Prof. Heinz
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Neumann, head of a work group specializing in Synthetic Biology, and Prof. Jörg Stülke from the department of General and Applied Microbiology.
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The next task was to find a project that was interesting as well as realizable. After a lot of back and forth due to extensive discussions, Prof.
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Neumann presented the perfect project: "Homing Coli"!
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<body class="mediawiki ns-0 ltr page-Team_Goettingen">
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After many drawbacks, like finding a suitable laboratory to realize the project, the search for additional team members began. The new
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fellows were quickly found, which was the story starting point of the first iGEM team of the University of Göttingen… Below you can see our timeline.
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[[File:Timeline.png|600px|thumb|center|The timeline of the iGEM Team Göttingen 2012. For a high resultion click on the picture.]]
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If you are interested in the [[Team:Goettingen/iGEM|iGEM competition]], the BioBrick concept, and – most importantly – [[Team:Goettingen/Project|our project]], take a look around. Here, you will find everything
 +
you need to know about "Homing Coli" and you will have the opportunity to gather knowledge about chemotaxis in general!
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Visit our [[Team:Goettingen/Team|team site]] to learn more about us and our supporters and advisors!
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And, last but not least, do not forget to check out our mascot  [[Team:Goettingen/Human_Practice/Flash_coli|Flash Coli]]! He always has some interesting stories to tell and needs YOUR help to fight the horrible phages!
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                        <!-- start content -->
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Feel free to browse this page!
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== Homing Coli - a short introduction ==
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                                <!-- Text body -->
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<font face="Verdana" size="-1">
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<br>
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Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
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<br>
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<table id="toc" class="toc"><tbody><tr><td><div id="toctitle"><h2>Contents</h2> <span class="toctoggle">[<a href="javascript:toggleToc()" class="internal" id="togglelink">hide</a>]</span></div>
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<ul>
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<li class="toclevel-1"><a href="#Welcome"><span class="tocnumber">1</span> <span class="toctext">Welcome to iGEM G&ouml;ttingen</span></a></li>
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<li class="toclevel-1"><a href="#Homing_Coli"><span class="tocnumber">2</span> <span class="toctext">Homing Coli</span></a></li>
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<li class="toclevel-1"><a href="#Synthetic_Biology"><span class="tocnumber">3</span> <span class="toctext">Synthetic Biology</span></a></li>
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</ul>
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</td></tr></tbody></table>
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<br>
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<h2><b><a name="Welcome"></a>Welcome to iGEM G&ouml;ttingen</b></h2>
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<p align="justify" style="line-height:1.6em">
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iGEM is an international competition hosted by the MIT in Boston, USA, for undergraduate students
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of disciplines related to molecular biology. iGEM stands for International Genetically Engineered
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Machine competition. On the one hand, it targets to combine aspects of education and social
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collaboration among undergraduate students, on the other hand, it provides a library for
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standardized and interchangeable parts which can be used in living systems, particularly
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in model organisms like <i>E. coli</i>. Student groups from all over the world will receive a
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requested kit of biological parts, also called "biobricks", and work over the summer on
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an individual research project. Every year, the "Registry of Standard Biological Parts"
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is upgraded with further biobricks by the participating iGEM teams. These biobricks will
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be accessible to the iGEM community in the following years in order to use these parts for
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their own projects.  <br>
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<br>
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The iGEM competition started in 2003 as a course for students of the MIT only. 2011,
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already 165 universities from all over the world competed against each other. This year,
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it is the first participation of the University of G&ouml;ttingen, Germany. The iGEM competition
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is a great opportunity for students to gain and improve a multitude of skills that are
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necessary during a carrier as a molecular biologist. Such skills would be planning and
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organizing a project, including fund raising and team recruitment, coordination of lab
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work, designing experiments and working together as a team. Next to intensive lab work
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during the summer, also literature search and well-structured documentation of the
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experiments is crucial. Moreover, safety for humans and environment is key.<br>
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</p>
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<br>
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<h2><b><a name="Homing_Coli"></a>Homing Coli</b></h2>
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<p align="justify" style="line-height:1.6em">
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<i>Escherichia coli</i> is a commonly used bacterial model organism. It has lots of beneficial
<i>Escherichia coli</i> is a commonly used bacterial model organism. It has lots of beneficial
traits, e.g. a short generation time and it can be easily manipulated. Most <i>E. coli</i>
traits, e.g. a short generation time and it can be easily manipulated. Most <i>E. coli</i>
strains that are used in laboratories do not exhibit high motility. The crucial element for
strains that are used in laboratories do not exhibit high motility. The crucial element for
motility is the flagellum, which is rotated by a molecular motor within the cell wall.
motility is the flagellum, which is rotated by a molecular motor within the cell wall.
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Consequently, these are reduced in cultivated <i>E. coli</i> strains.<br>
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Consequently, these are reduced in cultivated <i>E. coli</i> strains.
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<br>
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Our goal is to create an <i>E. coli</i> strain with increased swimming motility on special
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Our goal is to create an <i>E. coli</i> strain with increased swimming motility on special agar plates. Therefore, we will overexpress regulators and parts of the <i>E. coli</i> flagellum in cultivated strains in order to enhance bacterial swimming ability. The fastest <i>E. coli</i> strains will be selected and further improved. At the same time we will then be able to create an effective motility-selection method.
-
agar plates. Therefore, we will overexpress regulators and parts of the <i>E. coli</i> flagellum in cultivated strains in order to enhance bacterial swimming ability. The fastest <i>E. coli</i> strains will be selected and
+
 
-
further improved. At the same time we will then be able to create an effective motility-selection
+
-
  method.         <br>
+
-
<br>
+
Now, you are probably wondering what the advantage of a fast <i> E. coli</i> might be. The
Now, you are probably wondering what the advantage of a fast <i> E. coli</i> might be. The
beneficial fast phenotype can be combined with the ability of this bacterium to sense
beneficial fast phenotype can be combined with the ability of this bacterium to sense
specific compounds in their environments. Chemoreceptors enable it to move towards
specific compounds in their environments. Chemoreceptors enable it to move towards
-
or along gradients of such substances. We will use PCR-based site directed mutagenesis on the <i> E. coli</i> TAR receptor. The combination of speed and chemotaxis allows
+
or along gradients of such substances. We will use PCR-based site directed mutagenesis on the <i> E. coli</i> Tar receptor. The combination of speed and chemotaxis allows
us to identify <i>E. coli</i> strains, which can sense interesting compounds. Thereby, an
us to identify <i>E. coli</i> strains, which can sense interesting compounds. Thereby, an
-
easy method for the detection of pollutants, toxins or even tumors could be provided.<br><br>
+
easy method for the detection of pollutants, toxins or even tumors could be provided.
-
<table cellpadding="3"><tr><td>
+
For detailled information see our <html><a href="https://2012.igem.org/Team:Goettingen/Project">project page</a></html>
-
<img width="400 px" src="http://www.patrickreinke.de/igem/homingcoli.jpg"></td>
+
-
<td valign="top"; cellpadding="30"><font size="-1"> <a href="http://jb.asm.org/content/186/22/7529.full"> <p align="justify">Clive S. Barker <i> et al. </i> (2004). Increased Motility of <i> Escherichia coli </i> by Insertion Sequence Element
+
-
Integration into the Regulatory Region of the flhD Operon. Journal of Bacteriology, Vol. 186: 7529-7537.</a> </font><br>
+
-
</td></tr>
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-
</table></p>
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-
<br>
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[[File:Homingcoli.jpg|600px|thumb|center|<html><a href="http://jb.asm.org/content/186/22/7529.full">Clive S. Barker <i> et al. </i> (2004). Increased Motility of <i> Escherichia coli </i> by Insertion Sequence Element Integration into the Regulatory Region of the flhD Operon. Journal of Bacteriology, Vol. 186: 7529-7537.</a></html>]]
-
<h2><b><a name="Synthetic_Biology"></a>Synthetic Biology</b></h2>
+
== Synthetic Biology ==
-
<p align="justify" style="line-height:1.6em">
 
Synthetic biology is an interdisciplinary scientific area that has recently developed.
Synthetic biology is an interdisciplinary scientific area that has recently developed.
It links various fields of science like biology, chemistry, physics, molecular genetics,
It links various fields of science like biology, chemistry, physics, molecular genetics,
-
informatics and engineering.<br>
+
informatics and engineering.
-
<br>
+
-
 
+
Due to this combination the relation of biological design and function can be investigated
Due to this combination the relation of biological design and function can be investigated
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modular design that facilitates their handling. The subsequent introduction of the synthetic
modular design that facilitates their handling. The subsequent introduction of the synthetic
constructs to living cells can either cause the replacement of original cellular components
constructs to living cells can either cause the replacement of original cellular components
-
or result in additional elements that act cooperatively or more or less autonomously (see fig. 1).<br>
+
or result in additional elements that act cooperatively or more or less autonomously (see fig. 1).
-
<br>
+
-
<img src="http://www.patrickreinke.de/igem/synbio.jpg">
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[[File:SynbioGoe.jpg|600px|thumb|center|After Nandagopaland and Elowitz (2011). A continuum of synthetic biology. Wild-type cells (<b>A</b>)
-
<br>
+
can be subject to two basic types of synthetic manipulation. (<b>B</b>) Autonomous synthetic circuits, consisting of
-
<font size="-1">After Nandagopaland and Elowitz (2011). A continuum of synthetic biology. Wild-type cells (<b>A</b>) can be subject to two basic types of synthetic manipulation. (<b>B</b>) Autonomous synthetic circuits, consisting of ectopic components, may be introduced into the cell. Such circuits process inputs and implement functions (red arrows) seperate from the endogenous circuitry (black). However, unknown interactions with the host cell may affect their function (purple arrows) (<b>C</b>)An alternative is to rewire (red lines) the endogenous circuits themselves to have new connectivity. (<b>D</b>) Extending this line of synthetic manipulation, synthetic circuits could be integrated into appropriately rewired endogenous circuitry to act as sensors and to implent additional functionality. Ultimate goals of this program are to be able to design and construct (<b>E</b>) synthetic circuits that can functionality replace endogenous circuits or (<b>F</b>) fully autonomous circuits that operate independently of the cellular mileu.</font>
+
ectopic components, may be introduced into the cell. Such circuits process inputs and implement functions (red arrows)
 +
seperate from the endogenous circuitry (black). However, unknown interactions with the host cell may affect their function
 +
(purple arrows) (<b>C</b>)An alternative is to rewire (red lines) the endogenous circuits themselves to have new connectivity.
 +
(<b>D</b>) Extending this line of synthetic manipulation, synthetic circuits could be integrated into appropriately rewired
 +
endogenous circuitry to act as sensors and to implent additional functionality. Ultimate goals of this program are to be
 +
able to design and construct (<b>E</b>) synthetic circuits that can functionality replace endogenous circuits or (<b>F</b>)
 +
fully autonomous circuits that operate independently of the cellular mileu. <html><a href="http://www.its.caltech.edu/~haylab/research/SBReview2011.pdf">Nagarajan Nandagopal and Michael B. Elowitz. (2011). Synthetic Biology: Integrated Gene Circuits. SCIENCE, Vol. 333: 1244-1248.</a></html>]]
 +
Another very important and necessary feature of biological parts is orthogonality, which means in this context that independent devices can be combined unrestrictedly.
 +
This principle derives from the area of engineering and aims to alter subsystems, without impeding others.
-
        <font size="-1">  <a href="http://www.its.caltech.edu/~haylab/research/SBReview2011.pdf">Nagarajan Nandagopal and Michael B. Elowitz. (2011). Synthetic Biology: Integrated
 
-
Gene Circuits. SCIENCE, Vol. 333: 1244-1248.</a> </font><br>
 
-
<br>
 
-
 
-
Another very important and necessary feature of biological parts is orthogonality,
 
-
which means in this context that independent devices can be combined unrestrictedly.
 
-
  This principle derives from the area of engineering and aims to alter subsystems,
 
-
  without impeding others.<br>
 
-
<br>
 
This way cells can be modified as requested, resulting in a predictable behaviour.
This way cells can be modified as requested, resulting in a predictable behaviour.
Among others, this technique can be beneficial for the effective production of
Among others, this technique can be beneficial for the effective production of
certain substances, like biofuels or antibiotics.
certain substances, like biofuels or antibiotics.
-
 
+
<html><center>
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</p>
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-
<br>
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<br>
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<body id="top">
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<a href="#top">&uarr; Return to top</a>
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<br>
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<br>
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<table bordercolor="black" border="1 px" width="600 px"><tr><td>
<table bordercolor="black" border="1 px" width="600 px"><tr><td>
<b>Important pages</b>:<br>
<b>Important pages</b>:<br>
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<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
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<a href="https://2012.igem.org/Team:Goettingen/Safety">Safety</a>;
<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
</td></tr>
</td></tr>
</table>
</table>
 +
</center></html>
 +
| style="width:240px; vertical-align:top" |
 +
<html>
 +
<h2><hr><span><b>Flash Coli</b></span></h2>
 +
<!-- News cap end -->
 +
<embed src="http://www.patrickreinke.de/igem/motionflashcoli.swf" type="application/x-shockwave-flash"
 +
pluginspage="http://www.macromedia.com/go/getflashplayer" wmode="transparent" height="180" width="240">
 +
<font face="Verdana" size="-1">To explore our project with Flash Coli click
 +
<a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">here</a>.<br>
 +
<!-- News cap -->
 +
<h2><hr><span><b>News</b></span></h2>
 +
<!-- News cap end -->
 +
<font align="left">
 +
<!-- News 22 end--><br><hr>
 +
<p align="left"><i>27 September 2012,</i>  <b>4 h to go!!!</b> <br>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#22">To the news</a>
 +
<br>
 +
<!-- News 22 end--><br>
 +
<br>
 +
<!-- News 21 end--><br><hr>
 +
<p align="left"><i>26 September 2012,</i>  <b>Results are online!</b> <br>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#21">To the news</a>
 +
<br>
 +
<!-- News 21 end--><br><hr> 
 +
<br>
 +
<!-- News 20 -->
 +
<p align="left"><i>25 September 2012,</i>  <b>BioBricks are on their way!</b> <br>
 +
</a>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#20">To the news</a>
 +
<br>
 +
<!-- News 20 end--><br><hr>
 +
   
 +
<!-- News 19.5 -->
 +
<p align="left"><i>22 September 2012,</i>  <b>First youtube videos are online!</b> <br>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#19.5">To the news</a>
 +
<br>
 +
<!-- News 19.5 end--><br><hr>
 +
<!-- News 19 -->
 +
<p align="left"><i>13 September 2012,</i>  <b>13 days to go!</b> <br>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#19">To the news</a>
 +
<br>
 +
        <!-- News 19 end--><br><hr>
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          <!-- News 18 -->
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<p align="left"><i>07 September 2012,</i> <b>We organized the "Legoland for Scientists" symposium at the University of Göttingen.
-
</font>
+
You can read everything about it <a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion">here.</a></b>
-
                                <!-- Text body zu Ende -->
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-
                                <!-- News body anfang -->
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-
</td><td width="250px">
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-
<p>
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-
<p>
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-
<font face="Verdana" size="-1">
+
-
 
+
-
 
+
-
 
+
</p>
</p>
-
<!-- News cap -->
+
<a href="https://2012.igem.org/Team:Goettingen/News#18">To the news</a>
-
<hr><h2> <span><b>Flash Coli</b></span></h2>
+
<br>
-
<!-- News cap end -->
+
        <!-- News 18 end--><br><hr>
-
<embed src="http://www.patrickreinke.de/igem/motionflashcoli.swf" type="application/x-shockwave-flash" pluginspage="http://www.macromedia.com/go/getflashplayer" wmode="transparent" height="180" width="240">
+
-
<font face="Verdana" size="-1">To explore our project with Flash Coli click
+
-
<a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">here</a>.
+
-
</font></p>
+
-
<br><hr>
+
-
<p>
+
-
</p><p>
+
-
<font face="Verdana" size="-1">
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-
 
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 +
<!-- News 17 -->
 +
<p align="left"><i>06 September 2012,</i>  <b>Only one day left until the information evening "Legoland for scientists"...</b> <br></a>
</p>
</p>
-
<!-- News cap -->
+
<a href="https://2012.igem.org/Team:Goettingen/News#17">To the news</a>
-
<h2> <span><b>News</b></span></h2>
+
<br>
-
<!-- News cap end -->
+
 +
        <!-- News 17 end--><br><hr>
-
<font align="left">
 
-
<br>
 
           <!-- News 16 -->
           <!-- News 16 -->
-
<p align="left"><i>30 August 2012,</i>  <b>The first version of 'Flash Coli - the game' is online now. click <a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">here</a></b>
+
<p align="left"><i>30 August 2012,</i>  <b>The first version of "Flash Coli - the game" is now online. Click
 +
<a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">here.</a></b>
</p>
</p>
-
<a href="https://2012.igem.org/Team:Goettingen/News#15">To the news</a><br>
+
<a href="https://2012.igem.org/Team:Goettingen/News#16">To the news</a><br>
         <!-- News 16 end--><br><hr>
         <!-- News 16 end--><br><hr>
           <!-- News 15 -->
           <!-- News 15 -->
-
<p align="left"><i>25 August 2012,</i>  <b>Today is the "synthetic biology" day in germany!</b>
+
<p align="left"><i>25 August 2012,</i>  <b>Today is the "Synthetic Biology" day in Germany!</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#15">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#15">To the news</a><br>
Line 704: Line 175:
           <!-- News 14 -->
           <!-- News 14 -->
-
<p align="left"><i>15 August 2012,</i>  <b>In our weekly seminar Anna told us an example for synthetic Biology</b>
+
<p align="left"><i>15 August 2012,</i>  <b>In our weekly seminar Anna presented an example for Synthetic Biology.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#14">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#14">To the news</a><br>
         <!-- News 14 end--><br><hr>
         <!-- News 14 end--><br><hr>
-
 
-
 
           <!-- News 13 -->
           <!-- News 13 -->
-
<p align="left"><i>08 August 2012,</i>  <b>Ali and Sandra conducted their presentations in our weekly seminar</b>
+
<p align="left"><i>08 August 2012,</i>  <b> Survey: Ready to be asked? </b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#13">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#13">To the news</a><br>
         <!-- News 13 end--><br><hr>
         <!-- News 13 end--><br><hr>
 +
        <!-- News 12.75 -->
 +
<p align="left"><i>01 August 2012,</i>  <b>Ali and Sandra conducted their presentations in our weekly seminar.</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#12.75">To the news</a><br>
 +
        <!-- News 12.75 end--><br><hr>
 +
        <!-- News 12.5 -->
 +
<p align="left"><i>19 August 2012,</i>  <b>Human practice task force established!</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#12.5">To the news</a><br>
 +
        <!-- News 12.5 end--><br><hr>
           <!-- News 12 -->
           <!-- News 12 -->
-
<p align="left"><i>12 July 2012,</i>  <b>In today’s weekly meeting Christian told us about the European teacher meet-up in Paris</b>
+
<p align="left"><i>12 July 2012,</i>  <b>In today’s weekly meeting Christian told us about the European teacher meet-up in Paris.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#12">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#12">To the news</a><br>
         <!-- News 12 end--><br><hr>
         <!-- News 12 end--><br><hr>
-
 
+
      <!-- News 11.5 -->
 +
<p align="left"><i>04 July 2012,</i>  <b>Let there be light!</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#11.5">To the news</a><br>
 +
        <!-- News 11.5 end--><br><hr>
           <!-- News 11 -->
           <!-- News 11 -->
-
<p align="left"><i>28 June 2012,</i>  <b>Anna, Corinna and Erik participated in the third annual congress for strategy processes ”Biotechnology 2020+”</b>
+
<p align="left"><i>28 June 2012,</i>  <b>Anna, Corinna and Erik participated in the third annual congress for strategy processes "Biotechnology 2020+".</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#11">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#11">To the news</a><br>
         <!-- News 11 end--><br><hr>
         <!-- News 11 end--><br><hr>
-
 
+
      <!-- News 10.5 -->
 +
<p align="left"><i>26 June 2012,</i>  <b><i>E. coli</i> strain spurred!</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#10.5">To the news</a><br>
 +
        <!-- News 10.5 end--><br><hr>
           <!-- News 10 -->
           <!-- News 10 -->
-
<p align="left"><i>21 June 2012,</i>  <b>Our first poster is designed</b>
+
<p align="left"><i>21 June 2012,</i>  <b>Our first poster is designed!</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#10">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#10">To the news</a><br>
         <!-- News 10 end--><br><hr>
         <!-- News 10 end--><br><hr>
-
 
+
        <!-- News 9.5 -->
 +
<p align="left"><i>13 June 2012,</i>  <b>Task: Find a human practice project!</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#9.5">To the news</a><br>
 +
        <!-- News 9.5 end--><br><hr>
           <!-- News 9 -->
           <!-- News 9 -->
-
<p align="left"><i>06 June 2012,</i>  <b>Thomas Maschner from LMU Munich visited us</b>
+
<p align="left"><i>06 June 2012,</i>  <b>Thomas Maschner, advisor from the iGEM team of the LMU Munich, came to visit us.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#9">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#9">To the news</a><br>
         <!-- News 9 end--><br><hr>
         <!-- News 9 end--><br><hr>
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+
        <!-- News 8.5 -->
 +
<p align="left"><i>01 June 2012,</i>  <b>Discussion of the first lab results</b> 
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#8.5">To the news</a><br>
 +
        <!-- News 8.5 end--><br><hr>
           <!-- News 8 -->
           <!-- News 8 -->
-
<p align="left"><i>15 May 2012,</i>  <b>The first picture of our complete Team</b>
+
<p align="left"><i>15 May 2012,</i>  <b>The first picture of our complete team.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#8">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#8">To the news</a><br>
         <!-- News 8 end--><br><hr>
         <!-- News 8 end--><br><hr>
-
 
-
 
           <!-- News 7 -->
           <!-- News 7 -->
-
<p align="left"><i>11 May 2012,</i>  <b>iGEM Göttingen is in the local Newspaper</b>
+
<p align="left"><i>11 May 2012,</i>  <b>iGEM Göttingen is in the local newspaper.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#7">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#7">To the news</a><br>
         <!-- News 7 end--><br><hr>
         <!-- News 7 end--><br><hr>
-
 
-
 
         <!-- News 6 --> <br>
         <!-- News 6 --> <br>
-
<p align="left"><i>04 May 2012,</i>  <b><a href="http://www.kws.com/">KWS SAAT AG</a> is now Head Sponsor</b>
+
<p align="left"><i>04 May 2012,</i>  <b><a href="http://www.kws.com/">KWS SAAT AG</a> is now Head Sponsor.</b>
</p>
</p>
<p align="left">
<p align="left">
Line 774: Line 265:
<a href="https://2012.igem.org/Team:Goettingen/News#6">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#6">To the news</a><br>
         <!-- News 6 end--><br><hr>
         <!-- News 6 end--><br><hr>
-
 
-
 
         <!-- News 5 -->
         <!-- News 5 -->
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<p align="left"><i>02 May 2012,</i>  <b>Today Anna presented our iGEM Logo</b>
+
<p align="left"><i>02 May 2012,</i>  <b>Today Anna presented our iGEM logo.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#5">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#5">To the news</a><br>
         <!-- News 5 end--><br><hr>
         <!-- News 5 end--><br><hr>
-
 
-
 
         <!-- News 4 -->
         <!-- News 4 -->
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<p align="left"><i>30 April 2012,</i>  <b>We inspect the Laboratory at the Max Planck Campus</b>
+
<p align="left"><i>30 April 2012,</i>  <b>We inspect the laboratory at the Max Planck campus.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#4">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#4">To the news</a><br>
         <!-- News 4 end--><br><hr>
         <!-- News 4 end--><br><hr>
-
 
-
 
         <!-- News 3 -->
         <!-- News 3 -->
-
<p align="left"><i>26 April 2012,</i>  <b>Weekly meeting: PCR-Lecture from Heinz</b>
+
<p align="left"><i>26 April 2012,</i>  <b>Weekly meeting: PCR-Lecture from Heinz.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#3">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#3">To the news</a><br>
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<p align="left"><i>19 April 2012,</i>  <b>The iGEM Team Göttingen 2012 is now complete!</b>
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<p align="left"><i>19 April 2012,</i>  <b>The iGEM team Göttingen 2012 is now complete!</b>
</p>
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<a href="https://2012.igem.org/Team:Goettingen/News#2">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#2">To the news</a><br>
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</p><p><i>04 April 2012,</i>    <b> <a href="http://www.sartorius.com/">Sartorius Stedim Biotech GmbH</a> is now Premium Sponsor</b>
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<p><i>04 April 2012,</i>    <b> <a href="http://www.sartorius.com/">Sartorius Stedim Biotech GmbH</a> is now Premium Sponsor.</b>
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<a href="https://2012.igem.org/Team:Goettingen/News#1">To the news</a><br><br><hr>
<a href="https://2012.igem.org/Team:Goettingen/News#1">To the news</a><br><br><hr>
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Latest revision as of 01:02, 27 September 2012

Deutsch  / English 

Contents

Welcome to iGEM Göttingen

In November 2011, most of us heard about iGEM competition for the first time. Erik, inspired by his former colleagues' enthusiastic reports, was looking for like-minded people at his new university that were willing to organize a whole project in the field of Synthetic Biology. He described the idea of the contest to anybody willing to listen. It did not take long to find five other interested students. Thus, the so called organization task force of the first iGEM team of the University of Göttingen was formed.

Luckily, the iGEM competition was already well known among the University´s professors and the team found their first supporters: Prof. Heinz Neumann, head of a work group specializing in Synthetic Biology, and Prof. Jörg Stülke from the department of General and Applied Microbiology. The next task was to find a project that was interesting as well as realizable. After a lot of back and forth due to extensive discussions, Prof. Neumann presented the perfect project: "Homing Coli"!

After many drawbacks, like finding a suitable laboratory to realize the project, the search for additional team members began. The new fellows were quickly found, which was the story starting point of the first iGEM team of the University of Göttingen… Below you can see our timeline.

The timeline of the iGEM Team Göttingen 2012. For a high resultion click on the picture.

If you are interested in the iGEM competition, the BioBrick concept, and – most importantly – our project, take a look around. Here, you will find everything you need to know about "Homing Coli" and you will have the opportunity to gather knowledge about chemotaxis in general!

Visit our team site to learn more about us and our supporters and advisors! And, last but not least, do not forget to check out our mascot Flash Coli! He always has some interesting stories to tell and needs YOUR help to fight the horrible phages!

Feel free to browse this page!

Homing Coli - a short introduction

Escherichia coli is a commonly used bacterial model organism. It has lots of beneficial traits, e.g. a short generation time and it can be easily manipulated. Most E. coli strains that are used in laboratories do not exhibit high motility. The crucial element for motility is the flagellum, which is rotated by a molecular motor within the cell wall. Consequently, these are reduced in cultivated E. coli strains.

Our goal is to create an E. coli strain with increased swimming motility on special agar plates. Therefore, we will overexpress regulators and parts of the E. coli flagellum in cultivated strains in order to enhance bacterial swimming ability. The fastest E. coli strains will be selected and further improved. At the same time we will then be able to create an effective motility-selection method.

Now, you are probably wondering what the advantage of a fast E. coli might be. The beneficial fast phenotype can be combined with the ability of this bacterium to sense specific compounds in their environments. Chemoreceptors enable it to move towards or along gradients of such substances. We will use PCR-based site directed mutagenesis on the E. coli Tar receptor. The combination of speed and chemotaxis allows us to identify E. coli strains, which can sense interesting compounds. Thereby, an easy method for the detection of pollutants, toxins or even tumors could be provided.

For detailled information see our project page

Clive S. Barker et al. (2004). Increased Motility of Escherichia coli by Insertion Sequence Element Integration into the Regulatory Region of the flhD Operon. Journal of Bacteriology, Vol. 186: 7529-7537.

Synthetic Biology

Synthetic biology is an interdisciplinary scientific area that has recently developed. It links various fields of science like biology, chemistry, physics, molecular genetics, informatics and engineering.

Due to this combination the relation of biological design and function can be investigated from an entirely new perspective. The previous approach was limited to the examination of a structure and its function and the attempt to explain how they correlate. Recently, the reverse strategy is applied. Biological parts are specifically designed and constructed according to a desired function. These parts are characterized by a standardized modular design that facilitates their handling. The subsequent introduction of the synthetic constructs to living cells can either cause the replacement of original cellular components or result in additional elements that act cooperatively or more or less autonomously (see fig. 1).

After Nandagopaland and Elowitz (2011). A continuum of synthetic biology. Wild-type cells (A) can be subject to two basic types of synthetic manipulation. (B) Autonomous synthetic circuits, consisting of ectopic components, may be introduced into the cell. Such circuits process inputs and implement functions (red arrows) seperate from the endogenous circuitry (black). However, unknown interactions with the host cell may affect their function (purple arrows) (C)An alternative is to rewire (red lines) the endogenous circuits themselves to have new connectivity. (D) Extending this line of synthetic manipulation, synthetic circuits could be integrated into appropriately rewired endogenous circuitry to act as sensors and to implent additional functionality. Ultimate goals of this program are to be able to design and construct (E) synthetic circuits that can functionality replace endogenous circuits or (F) fully autonomous circuits that operate independently of the cellular mileu. Nagarajan Nandagopal and Michael B. Elowitz. (2011). Synthetic Biology: Integrated Gene Circuits. SCIENCE, Vol. 333: 1244-1248.

Another very important and necessary feature of biological parts is orthogonality, which means in this context that independent devices can be combined unrestrictedly. This principle derives from the area of engineering and aims to alter subsystems, without impeding others.

This way cells can be modified as requested, resulting in a predictable behaviour. Among others, this technique can be beneficial for the effective production of certain substances, like biofuels or antibiotics.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Safety; Attributions

Flash Coli

To explore our project with Flash Coli click here.

News


27 September 2012, 4 h to go!!!

To the news



26 September 2012, Results are online!

To the news


25 September 2012, BioBricks are on their way!

To the news

22 September 2012, First youtube videos are online!

To the news

13 September 2012, 13 days to go!

To the news

07 September 2012, We organized the "Legoland for Scientists" symposium at the University of Göttingen. You can read everything about it here.

To the news

06 September 2012, Only one day left until the information evening "Legoland for scientists"...

To the news

30 August 2012, The first version of "Flash Coli - the game" is now online. Click here.

To the news

25 August 2012, Today is the "Synthetic Biology" day in Germany!

To the news

15 August 2012, In our weekly seminar Anna presented an example for Synthetic Biology.

To the news

08 August 2012, Survey: Ready to be asked?

To the news

01 August 2012, Ali and Sandra conducted their presentations in our weekly seminar.

To the news

19 August 2012, Human practice task force established!

To the news

12 July 2012, In today’s weekly meeting Christian told us about the European teacher meet-up in Paris.

To the news

04 July 2012, Let there be light!

To the news

28 June 2012, Anna, Corinna and Erik participated in the third annual congress for strategy processes "Biotechnology 2020+".

To the news

26 June 2012, E. coli strain spurred!

To the news

21 June 2012, Our first poster is designed!

To the news

13 June 2012, Task: Find a human practice project!

To the news

06 June 2012, Thomas Maschner, advisor from the iGEM team of the LMU Munich, came to visit us.

To the news

01 June 2012, Discussion of the first lab results

To the news

15 May 2012, The first picture of our complete team.

To the news

11 May 2012, iGEM Göttingen is in the local newspaper.

To the news


04 May 2012, KWS SAAT AG is now Head Sponsor.

To the news

02 May 2012, Today Anna presented our iGEM logo.

To the news

30 April 2012, We inspect the laboratory at the Max Planck campus.

To the news

26 April 2012, Weekly meeting: PCR-Lecture from Heinz.

To the news

19 April 2012, The iGEM team Göttingen 2012 is now complete!

To the news


04 April 2012, Sartorius Stedim Biotech GmbH is now Premium Sponsor.

To the news

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