Team:LMU-Munich/Data

From 2012.igem.org

(Difference between revisions)
 
(132 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}}
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}}
 +
[[File:Data banner.resized WORDS.JPG|620px|link=]]
-
==Data==
 
-
On this page, we will upload data to explain our project progress. More to come!!!
+
[[Image:BacillusBioBrickBox.png|right|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
 +
<br>
 +
<div class="box" style= "background-color:#e4f1d7">
-
Or see the results pages of our projects to follow our progress:
+
==='''''Bacillus''B'''io'''B'''rick'''B'''ox===
-
{|
+
 
-
|<font color="#FFFFFF">+--+--+--+--+--+--+--</font>
+
</div>
-
|<font color="#FFFFFF">+--+--+--+--+--</font>
+
<div class="box">
-
|<font color="#FFFFFF">+--+--+--+--+--+--</font>
+
====Evaluation of ''B. subtilis'' vectors====
-
|<font color="#FFFFFF">+--+--+--+--+--+--</font>
+
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">Seven novel vectors were constructed and four already proven to work in ''B. subtilis''.</p>
 +
|[[File:LacZ plate.png|right|100px|link=Team:LMU-Munich/Data/Vectors]]
|-
|-
-
|
+
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Vectors]]
-
<html>
+
|}
-
<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
+
</div>
-
<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=70"/>
+
<div class="box">
-
</a>
+
====Anderson Promoter Evaluation====
-
<font size="1">
+
{| "width=100%" style="text-align:center;"|
-
</font>
+
|<p align="justify">Eleven Anderson promoters were measured in ''B. subtilis'' and showed relatively weak activity.</p>
-
<br />
+
|[[File:LMU Anderson preview.jpg|200px|link=Team:LMU-Munich/Data/Anderson]]
-
<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction"><font size="1" face="verdana">Bacillus<BR>Intro</font></a>
+
|-
-
</html>
+
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Anderson]]
 +
|}
 +
</div>
 +
<div class="box">
 +
====Constitutive ''Bacillus'' Promoters====
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks (P<sub>''liaG''</sub>, P<sub>''veg''</sub>, P<sub>''lepA''</sub>) were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
 +
|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Constitutive]]
 +
|}
 +
</div>
 +
<div class="box">
 +
====Inducible ''Bacillus'' Promoters====
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">A novel antibiotic-inducible promoter, P<sub>''liaI''</sub>, was constructed and thoroughly evaluated.</p>
 +
|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Inducible]]
 +
|}
 +
</div>
-
|<html>
+
[[File:SporeCoat.png|90px|right|link=Team:LMU-Munich/Spore_Coat_Proteins]]
-
<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
+
<br>
-
<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=70"/>
+
<div class="box" style= "background-color:#e4f1d7">
-
</a>
+
=='''Sporo'''beads==
-
<font size="1">
+
</div>
-
</font>
+
-
<br />
+
-
<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks"><font size="1" face="verdana">Bacillus<BR>BioBrickBOX</font></a>
+
-
</html>
+
-
|<html>
+
<div class="box">
-
<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
+
===Crust Promoter Evaluation===
-
<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=70"/>
+
{| "width=100%" style="text-align:center;"|
-
</a>
+
|<p align="justify">Three sporulation-dependent promoters (P<sub>''cotYZ''</sub>, P<sub>''cotV''</sub>, P<sub>''cgeA''</sub>) were constructed, two of which show the expected behavior.</p>
-
<font size="1">
+
|[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
-
</font>
+
|-
-
<br />
+
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/crustpromoters]]
-
<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins"><font size="1" face="verdana">SporeCoat<BR>FusionProteins</font></a>
+
|}
-
</html>
+
</div>
-
|<html>
+
<div class="box">
-
<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
+
===GFP-'''Sporo'''bead Evaluation===
-
<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=70"/>
+
{| "width=100%" style="text-align:center;"|
-
</a>
+
|<p align="justify">They glow! GFP was successfully displayed on the spore crust as demonstrated by fluorescence microscopy.</p>
-
<font size="1">
+
|[[File:Sporobead Constructs Dada sheet preview.png|200px|link=Team:LMU-Munich/Data/gfp_spore]]
-
</font>
+
|-
-
<br />
+
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/gfp_spore]]
-
<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"><font size="1" face="verdana">Germination<BR>STOP</font></a>
+
-
</html>
+
|}
|}
 +
</div>
-
asdasd<asd
+
<div class="box">
-
[[File:Auswertung Anderson promoters.png|thumb|right|Fig. 1: luminescence measurement of Anderson promoters in the reporter vector pSB<sub>''Bs''</sub>3C-''luxABCDE''. OD<sub>''600''</sub> (right), LUMI (center) and OD<sub>''600''</sub> per LUMI (left) depending on the time (h). Data come from three independant experiments. Curves were fitted over each other (t=0, OD<sub>''600''</sub>=0,3) and smothed by taking average of three neighbouring values.]]
+
==='''Sporo'''bead Purification===
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells: Lysozyme is the best!</p>
 +
|[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Sporepurification]]
 +
|}
 +
</div>
 +
[[File:GerminationSTOP.png|90px|right|link=Team:LMU-Munich/Germination_Stop]]
 +
<br>
 +
<div class="box" style= "background-color:#e4f1d7">
 +
==='''Germination'''STOP===
 +
</div>
 +
<div class="box">
 +
===Knockouts of germination genes ===
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">We successfully prevented spore germination by multiple gene knockouts.</p>
 +
|[[File:LMU Knockout previewii.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Knockout]]
 +
|}
 +
</div>
 +
<div class="box">
 +
==='''Suicide'''Switch ===
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">Death upon germination: We developed a novel safety strategy as a back-up to the gene knockouts.</p>
 +
|[[File:PlatereaderSuicideSwitch1.jpg|200px|link=Team:LMU-Munich/Data/Suicideswitch]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Suicideswitch]]
 +
|}
 +
</div>
 +
 +
[[File:LMU-Munich-Invertersign.png|90px|right|link=Team:LMU-Munich/Inverter]]
 +
<br>
 +
<div class="box" style= "background-color:#e4f1d7">
 +
===Inverter===
 +
</div>
 +
 +
<div class="box">
 +
===β-galactosidase assay of Inverter with ''lacZα'' ===
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">It works! Invert the output of your promoter of choice.</p>
 +
|[[File:LMU Inverter graph.png|200px|link=Team:LMU-Munich/Data/Inverter]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Inverter]]
 +
|}
 +
</div>
 +
 +
 +
<div class="box" style= "background-color:#e4f1d7">
 +
==='''Trash'''can===
 +
Things that were a pain in the ''neck''!
 +
</div>
 +
 +
<div class="box">
 +
===''cge''A and P<sub>''cge''A</sub>===
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">
 +
Unexpected restriction sites combined with no activity at all - what a bummer!</p>
 +
|[[File:LMU cgea preview.jpg|200px|link=Team:LMU-Munich/Data/cgeA]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/cgeA]]
 +
|}
 +
</div>
 +
 +
<div class="box">
 +
===MazF===
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">
 +
The story of trying to clone a toxin from ''E. coli'' in ''E. coli'': They die, dammit! (...not a surprise, really...)
 +
</p>
 +
|[[File:MazFtrashcan.jpg|200px|link=Team:LMU-Munich/Data/MazF]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/MazF]]
 +
|}
 +
</div>
 +
 +
<div class="box">
 +
===Xylose promoters===
 +
{| "width=100%" style="text-align:center;"|
 +
|<p align="justify">
 +
All constructs involving xylose-inducible promoters refused cloning or evaluation (sorry, Groningen...!).
 +
</p>
 +
|[[File:100px-D-Xylose Keilstrich.png|100px|link=Team:LMU-Munich/Data/Pxyl]]
 +
|-
 +
! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Pxyl]]
 +
|}
 +
</div>
 +
 +
<p></p>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
For our big breakthroughs, or to follow specific projects, see the individual project pages:
 +
 +
 +
<div class="box">
 +
====Project Navigation====
 +
{| width="100%" align="center" style="text-align:center;"
 +
|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
 +
|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
 +
|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
 +
|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
 +
|-
 +
|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
 +
|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
 +
|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
 +
|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
 +
|}
 +
</div>
{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 14:31, 22 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo2.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Data"