Team:WashU/CharacterizationOther

From 2012.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 2: Line 2:
{{WashUprojectbar}}
{{WashUprojectbar}}
<html>
<html>
-
<div id = "zconstructchar">
+
<div id = "phaseIII">
-
<h1>Phase II</h1>
+
<h1>Phase III</h1>
-
<p>
+
<p>
-
The purpose of Phase II is to produce a set of biobricks that properly produce the proteins that wil be put together to react and turn zeaxanthin into the final saffron components. Thus, this phase deals with expression of ZCD and UGTCS2 and characterization of those parts. In addition, to prepare for Phase III, we have done some basic characterization of crocin, which will be used for characterization in Phase III when optimizing the production of safranal and crocin.
+
The purpose of Phase III is to complete the production of safranal and crocin by putting together the biobricks from Phase II.In anticipation for the final biobrick which will contain all of our genes together in one plasmid, we created a variety of standardization curves to compare yield and calculate important constants. For example, we made a standard curve of crocin in water to calculate its extinction coefficient.  
</p>
</p>
<img src="http://dl.dropbox.com/u/88390549/CrocinGraphs2.png"> <br><br>
<img src="http://dl.dropbox.com/u/88390549/CrocinGraphs2.png"> <br><br>
-
<img src="https://static.igem.org/mediawiki/igem.org/5/53/Ugtsds.png">
+
<p>
-
 
+
Using the trendline below, by the Beer-Lambert law, the extinction coefficient of crocin in water is 15258 M<sup>-1</sup> cm<sup>-1</sup>.  
-
We created a standard curve of crocin in water, in order to calculate the extinction coefficient. Using the trendline below, by the Beer-Lambert law, the extinction coefficient of crocin in water is 15258 M<sup>-1</sup> cm<sup>-1</sup>.  
+
</p>
<br>
<br>
-
<img src="http://dl.dropbox.com/u/88390549/ExtinctionCoefficientCrocin.png" width=750px>
+
<img src="http://dl.dropbox.com/u/88390549/Extinction%20Coefficient.png" width=750px>
 +
 
 +
 
</div>
</div>
</html>
</html>

Latest revision as of 00:05, 1 October 2012




Phase III

The purpose of Phase III is to complete the production of safranal and crocin by putting together the biobricks from Phase II.In anticipation for the final biobrick which will contain all of our genes together in one plasmid, we created a variety of standardization curves to compare yield and calculate important constants. For example, we made a standard curve of crocin in water to calculate its extinction coefficient.



Using the trendline below, by the Beer-Lambert law, the extinction coefficient of crocin in water is 15258 M-1 cm-1.


Retrieved from "http://2012.igem.org/Team:WashU/CharacterizationOther"