"

From 2012.igem.org

Revision as of 03:40, 28 August 2012 (view source) Ehoyer (Talk | contribs) ← Older edit		Latest revision as of 09:41, 4 September 2012 (view source) Ehoyer (Talk | contribs) (→Competent Cell Testing)
(2 intermediate revisions not shown)
Line 1:		Line 1:
	{{:Team:Macquarie_Australia/Template/MQ12}}		{{:Team:Macquarie_Australia/Template/MQ12}}
		+
		+	==='''Competent Cell Testing'''===
		+
		+	'''Results:'''
		+	* The transformation efficiency for all of the Top10 cells were 1 x 10^7 CFU/µg while the transofmration efficiency for the Fusion Blue and BL21 cells were 1 x 10^6 CFU/µg.
		+
		+	Recommendation:
		+	* New competent cells need to be made from the Top10 as we need at least a transformation efficiency of 1 x 10^8 CFU/µg.

---

Latest revision as of 09:41, 4 September 2012

Competent Cell Testing

Results:

• The transformation efficiency for all of the Top10 cells were 1 x 10^7 CFU/µg while the transofmration efficiency for the Fusion Blue and BL21 cells were 1 x 10^6 CFU/µg.

Recommendation:

• New competent cells need to be made from the Top10 as we need at least a transformation efficiency of 1 x 10^8 CFU/µg.