"
Team:Macquarie Australia/Protocols/CompetentCellTesting
From 2012.igem.org
(Difference between revisions)
(→Competent Cell Testing) |
|||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:Macquarie_Australia/Template/MQ12}} | {{:Team:Macquarie_Australia/Template/MQ12}} | ||
| - | + | ==='''Competent Cell Testing'''=== | |
| + | |||
| + | '''Results:''' | ||
| + | * The transformation efficiency for all of the Top10 cells were 1 x 10^7 CFU/µg while the transofmration efficiency for the Fusion Blue and BL21 cells were 1 x 10^6 CFU/µg. | ||
| + | |||
| + | Recommendation: | ||
| + | * New competent cells need to be made from the Top10 as we need at least a transformation efficiency of 1 x 10^8 CFU/µg. | ||
Latest revision as of 09:41, 4 September 2012
Competent Cell Testing
Results:
- The transformation efficiency for all of the Top10 cells were 1 x 10^7 CFU/µg while the transofmration efficiency for the Fusion Blue and BL21 cells were 1 x 10^6 CFU/µg.
Recommendation:
- New competent cells need to be made from the Top10 as we need at least a transformation efficiency of 1 x 10^8 CFU/µg.
