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Team:Grenoble/Biology/Protocols/Glycerol
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<h1>Glycerol stock preparation</h1> | <h1>Glycerol stock preparation</h1> | ||
<h2>Goal</h2> | <h2>Goal</h2> | ||
| - | + | Glycerol stock is a way to store bacteria for a long period. | |
</section> | </section> | ||
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You need to: | You need to: | ||
<ol> | <ol> | ||
| - | <li> | + | <li>Grow a liquid culture overnight.</li> |
| - | <li> | + | <li>Prepare sterile 80% glycerol.</li> |
| - | <li> | + | <li>Mix 200 µL of glycerol and 800 µL of liquid culture.</li> |
</ol> | </ol> | ||
<br/> | <br/> | ||
Latest revision as of 08:53, 28 August 2012
Glycerol stock preparation
Goal
Glycerol stock is a way to store bacteria for a long period.Protocol
You need to:- Grow a liquid culture overnight.
- Prepare sterile 80% glycerol.
- Mix 200 µL of glycerol and 800 µL of liquid culture.
Then store the glycerol stock at -80°C.
