Team:Groningen

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<l1 >Foodwarden</l1><br>
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<l2>'''The Food Warden: ''it's rotten and you know it!'' '''
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Every year, 1,300,000,000 tons of food are thrown away worldwide. This is one third of the global food production. One of the reasons for this is the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save up to 600 euro per household per year.
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Our goal is to build a system to sense meat spoilage. When a package of meat is saved after opening, a closed sticker containing ''Bacillus subtilis'' spores is activated by breaking the middle compartment, similar to activating a glow-in-the-dark stick, whereby alanine and water are mixed with the spores to cause germination. When the germinated microbes come in contact with volatiles from rotting meat, a pathway is activated which results to the production of a pigment. When this happens, the consumer knows that he has to throw away the food.  
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We use two strategies to make ''Bacillus subtilis'' react to rotten meat volatiles. The first involves TnrA, a transcription regulator involved in nitrogen metabolism. The picture below summerizes this goal pathway. The other strategy comprises the identification of new promoters reacting to rotten meat volatiles. In both cases, the promoter will be combined to a pigment production pathway. Besides that, iGEM Groningen 2012 aims to identify the volatiles that cause the promoter induction by using Gas Chromatography and Mass Spectrometry. A detailed description of our project plan can be found
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<z1 >Food Warden</z1><br>
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<img src="https://static.igem.org/mediawiki/2012/9/97/Groningen2012_AD20121915_Sticker.png" width="130" height="130" align=left style="padding-right: 10px; padding-top: 20px; padding-bottom:15px;"><a href="https://2012.igem.org/Team:Groningen/Project"><font color=#ff6700 size=4><b>The Food Warden</b></font></a><br>
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The Food Warden is a system which detects meat spoilage. It uses <i>Bacillus subtilis</i> cells. Their natural genetic response to the gases of rotten meat has been identified and linked to a pigment production system. In this way, a consumer can easily see when the meat is spoiled: <b><i>it's rotten and you know it!</i></b><br>
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<a href="https://2012.igem.org/Team:Groningen/Project"><font color=#ff6700 size=2>read more</font></a><br>
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<a href="https://2012.igem.org/Team:Groningen/Data_page"><font color=#ff6700 size=4><b>How does it work?</b></font></a><br>
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Volatiles from rotten meat activate the P<i><font size=2>sboA</font></i> promoter, triggering the production of pigments (blue or yellow).<br>More details can be found on our <a href=https://2012.igem.org/Team:Groningen/Data_page><font color=#ff6700><b>Data Page</b></font></a>.<br></p>
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<a href="https://2012.igem.org/Team:Groningen/Stop_the_food_waste_initiative"><font color=#ff6700 size=4><b>Stop the food waste!</b></font></a><br>
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<img src="https://static.igem.org/mediawiki/2012/f/f2/Groningen2012_AD20120915_StopFoodWaste.png" width="130" height="130" align=right style="padding-left: 5px; padding-right: 5px;">Every year, 1.3 billion tons of food are thrown away worldwide (one third of total food production), partly due to the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save money and reduce food waste.<br>
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<a href="https://2012.igem.org/Team:Groningen/Stop_the_food_waste_initiative"><font color=#ff6700 SIZE=2pt>read more</font></a><br><br><br>
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<font size = 4 color=#ff6700><b>Our achievements</b></font><br><br>
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<img src=https://static.igem.org/mediawiki/2012/1/16/Groningen2012_GoldMedal.png style="padding-bottom:3px;"><br><font color=#ffff00><b> Gold Medal</b></font><br><br>
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<img src="https://static.igem.org/mediawiki/2012/2/27/Groningen2012_ADTrophy_igem.png"><br>
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<b>Regional Winner</b><br>
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<font size=2>at the iGEM European Jamboree</font><br></p>
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<img src="https://static.igem.org/mediawiki/2012/c/c5/IGGroningen2012_Brick.png" height=50><br>
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<b>World Championship!!!</b><br>
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<a href="https://2012.igem.org/files/presentation/Groningen_Championship.pdf" target=_blank>
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Our presentation [.pdf]</a> ||
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<a href="https://2012.igem.org/files/video/Groningen_Championship.mp4" target=_blank>
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Our presentation (recording)</a> ||
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<a href="https://igem.org/images/easel_icon.png" target=_blank>
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Our poster</a>
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<z3 >Picture gallery</z3><br>
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'''The Food Warden: ''it's rotten and you know it!'' '''
 
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Every year, 1,300,000,000 tons of food are thrown away worldwide. This is one third of the global food production. One of the reasons for this is the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save up to 600 euro per household per year.
 
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Our goal is to build a system to sense meat spoilage. When a package of meat is saved after opening, a closed sticker containing ''Bacillus subtilis'' spores is activated by breaking the middle compartment, similar to activating a glow-in-the-dark stick, whereby alanine and water are mixed with the spores to cause germination. When the germinated microbes come in contact with volatiles from rotting meat, a pathway is activated which results to the production of a pigment. When this happens, the consumer knows that he has to throw away the food.
 
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We use two strategies to make ''Bacillus subtilis'' react to rotten meat volatiles. The first involves TnrA, a transcription regulator involved in nitrogen metabolism. The picture below summerizes this goal pathway. The other strategy comprises the identification of new promoters reacting to rotten meat volatiles. In both cases, the promoter will be combined to a pigment production pathway. Besides that, iGEM Groningen 2012 aims to identify the volatiles that cause the promoter induction by using Gas Chromatography and Mass Spectrometry. A detailed description of our project plan can be found [[Team:Groningen/Project|on the project page]].
 
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|[[File:JP 20120504 ReactionPathwayOverview v03.png|680px|center]]
 
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|Fig. h1. Overview of the functional reaction pathway for ''B. salus carnis''.
 
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<p align=center><font color=white size=2>Click on any of the list below to see the appropriate wiki section!</font></p>
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<p class="marginChecklist"><b>BRONZE</b>
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<a href="https://igem.org/Team.cgi" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Team registration done</a><br>
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<a href="https://igem.org/2012_Judging_Form?id=818" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Judging form completed</a><br>
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<a href="https://2012.igem.org/Team:Groningen" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Beautiful Team Wiki finished</a><br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We present our poster and project at iGEM Jamboree in Amsterdam at Saturday 6 October 2012<br>
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<a href="https://2012.igem.org/Team:Groningen/OurBiobrick" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Our Biobricks</a>
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<b>SILVER</b> (in addition to bronze medal requirements)
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<a href="http://partsregistry.org/Part:BBa_K818000" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Proof that our BioBrick works!</a><br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> <a href="http://partsregistry.org/Part:BBa_K818600" target=_blank>Characterization of our BioBrick's operation: BBa_K818600</a>
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<b>GOLD</b> (in addition to silver and bronze medal requirements, any one or more of the following)
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We improved the function of existing BioBricks: <a href="http://partsregistry.org/Part:BBa_K592010" target=_blank>BBa_K592010</a>, <a href="http://partsregistry.org/Part:BBa_K592009" target=_blank>BBa_K592009</a><br>
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<a href="https://2012.igem.org/Team:Groningen/international_cooperation#LMUMunich" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We helped iGEM LMU Munich by characterizing their parts</a><br>
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<a href="https://2012.igem.org/Team:Groningen/Safety" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We explored the topic of safety for GMOs that would be used near food</a><br>
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<a href="https://2012.igem.org/Team:Groningen/Stop_the_food_waste_initiative" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We raised public awareness about food waste in developed countries</a><br>
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<a href="https://2012.igem.org/Team:Groningen/market_research" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We surveyed the broad public about the need for Food Warden product and GMOs safety</a>
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Latest revision as of 14:21, 1 September 2013





Food Warden



The Food Warden
The Food Warden is a system which detects meat spoilage. It uses Bacillus subtilis cells. Their natural genetic response to the gases of rotten meat has been identified and linked to a pigment production system. In this way, a consumer can easily see when the meat is spoiled: it's rotten and you know it!
read more

How does it work?
Volatiles from rotten meat activate the PsboA promoter, triggering the production of pigments (blue or yellow).
More details can be found on our Data Page.

Stop the food waste!
Every year, 1.3 billion tons of food are thrown away worldwide (one third of total food production), partly due to the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save money and reduce food waste.
read more

Our achievements


Gold Medal


Regional Winner
at the iGEM European Jamboree


World Championship!!!

Our presentation [.pdf] || Our presentation (recording) || Our poster


Picture gallery





































Gold medal checklist

Click on any of the list below to see the appropriate wiki section!

BRONZE

Team registration done
Judging form completed
Beautiful Team Wiki finished
We present our poster and project at iGEM Jamboree in Amsterdam at Saturday 6 October 2012
Our Biobricks

SILVER (in addition to bronze medal requirements)

Proof that our BioBrick works!
Characterization of our BioBrick's operation: BBa_K818600

GOLD (in addition to silver and bronze medal requirements, any one or more of the following)

We improved the function of existing BioBricks: BBa_K592010, BBa_K592009
We helped iGEM LMU Munich by characterizing their parts
We explored the topic of safety for GMOs that would be used near food
We raised public awareness about food waste in developed countries
We surveyed the broad public about the need for Food Warden product and GMOs safety

Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen"