Team:Technion/21 August 2012

From 2012.igem.org

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==Inbal==
==Inbal==
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-Returning on the PCR from yesterday, But with different Tm's with the same primers (for pSB1C3 & pPROLAR separately) . The results: still not successful- there weren't any bands closed to 2700 bp. <br>
 +
The conclusion- We (Shahar, Evgeni, Rachel and I) need to plan new primers for the RNAP's PCR.
==Asaf==
==Asaf==
 +
-I ran the PCR products from yesterday on gel agarose to check what I got, and I got the expected bands (500 bp).<br>
 +
-I planned the PCR reaction for extracting the different RNA polymerase genes for the different plasmids with the <br> appropriate overhangs (T7*,N4,T3,K1F,SP6,T7 w.t).
==Hila==
==Hila==
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==Lior==
==Lior==
-
Today me and Noa did a second PCR run for Chriss's plasmids- k1f, T3, pN4, sp6. only the k1f came out good. we'll change the Tm for better result in the future PCR programs. <br> wev'e done transformation for the xyIE and ALP in Top10 cells and plated them on CM plate.
+
Today me and Noa did a second PCR run for Chris's plasmids- k1f, T3, pN4, sp6. only the k1f came out good. we'll change the Tm for better result in the future PCR programs. <br> we've done transformation for the xyIE and ALP in Top10 cells and plated them on CM plate.
==Noa==
==Noa==
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==Shahar==
==Shahar==
-
 
+
trying to do the PCR from yesterday again, But with different Tm's with the same primers (for pSB1C3 & pPROLAR separately) . The results: fail. there weren't any bands closed to 2700 bp. <br>
 +
conclusion- there are problems with the priners, and we need to plan new primers for the RNAP's PCR.
==Rachel==
==Rachel==
}}
}}

Latest revision as of 11:29, 30 August 2012



Team:Technion/21_August_2012
March
MTWTFSS
      [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_March_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_March_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_March_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_March_2012&preload=Team:Technion/Template:NewDate&action=edit 4]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/5_March_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_March_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_March_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_March_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_March_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_March_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_March_2012&preload=Team:Technion/Template:NewDate&action=edit 11]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/12_March_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_March_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_March_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_March_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_March_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_March_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_March_2012&preload=Team:Technion/Template:NewDate&action=edit 18]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/19_March_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_March_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/Team:Technion/21_March_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_March_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_March_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_March_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_March_2012&preload=Team:Technion/Template:NewDate&action=edit 25]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/26_March_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_March_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_March_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_March_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_March_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_March_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
April
MTWTFSS
            [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_April_2012&preload=Team:Technion/Template:NewDate&action=edit 1]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/2_April_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/Team:Technion/3_April_2012 3] [http://2012.igem.org/Team:Technion/4_April_2012 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_April_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_April_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_April_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_April_2012&preload=Team:Technion/Template:NewDate&action=edit 8]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/9_April_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_April_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_April_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_April_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_April_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/Team:Technion/14_April_2012 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_April_2012&preload=Team:Technion/Template:NewDate&action=edit 15]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/16_April_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_April_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_April_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_April_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_April_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_April_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_April_2012&preload=Team:Technion/Template:NewDate&action=edit 22]
[http://2012.igem.org/Team:Technion/23_April_2012 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_April_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/Team:Technion/25_April_2012 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_April_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_April_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/Team:Technion/28_April_2012 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_April_2012&preload=Team:Technion/Template:NewDate&action=edit 29]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/30_April_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
May
MTWTFSS
  [http://2012.igem.org/Team:Technion/1_May_2012 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_May_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_May_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_May_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_May_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_May_2012&preload=Team:Technion/Template:NewDate&action=edit 6]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/7_May_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_May_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_May_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/Team:Technion/10_May_2012 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_May_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_May_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_May_2012&preload=Team:Technion/Template:NewDate&action=edit 13]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/14_May_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_May_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_May_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_May_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_May_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_May_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_May_2012&preload=Team:Technion/Template:NewDate&action=edit 20]
[http://2012.igem.org/Team:Technion/21_May_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_May_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_May_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_May_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_May_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_May_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_May_2012&preload=Team:Technion/Template:NewDate&action=edit 27]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/28_May_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_May_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_May_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_May_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
June
MTWTFSS
        [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_June_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_June_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/Team:Technion/3_June_2012 3]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/4_June_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/Team:Technion/5_June_2012 5] [http://2012.igem.org/Team:Technion/6_June_2012 6] [http://2012.igem.org/Team:Technion/7_June_2012 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_June_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_June_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/Team:Technion/10_June_2012 10]
[http://2012.igem.org/Team:Technion/11_June_2012 11] [http://2012.igem.org/Team:Technion/12_June_2012 12] [http://2012.igem.org/Team:Technion/13_June_2012 13] [http://2012.igem.org/Team:Technion/14_June_2012 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_June_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_June_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/Team:Technion/17_June_2012 17]
[http://2012.igem.org/Team:Technion/18_June_2012 18] [http://2012.igem.org/Team:Technion/19_June_2012 19] [http://2012.igem.org/Team:Technion/20_June_2012 20] [http://2012.igem.org/Team:Technion/21_June_2012 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_June_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_June_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/Team:Technion/24_June_2012 24]
[http://2012.igem.org/Team:Technion/25_June_2012 25] [http://2012.igem.org/Team:Technion/26_June_2012 26] [http://2012.igem.org/Team:Technion/27_June_2012 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_June_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_June_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_June_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
July
MTWTFSS
            [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_July_2012&preload=Team:Technion/Template:NewDate&action=edit 1]
[http://2012.igem.org/Team:Technion/2_July_2012 2] [http://2012.igem.org/Team:Technion/3_July_2012 3] [http://2012.igem.org/Team:Technion/4_July_2012 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_July_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_July_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_July_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/Team:Technion/8_July_2012 8]
[http://2012.igem.org/Team:Technion/9_July_2012 9] [http://2012.igem.org/Team:Technion/10_July_2012 10] [http://2012.igem.org/Team:Technion/11_July_2012 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_July_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_July_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_July_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_July_2012&preload=Team:Technion/Template:NewDate&action=edit 15]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/16_July_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/Team:Technion/17_July_2012 17] [http://2012.igem.org/Team:Technion/18_July_2012 18] [http://2012.igem.org/Team:Technion/19_July_2012 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_July_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_July_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/Team:Technion/22_July_2012 22]
[http://2012.igem.org/Team:Technion/23_July_2012 23] [http://2012.igem.org/Team:Technion/24_July_2012 24] [http://2012.igem.org/Team:Technion/25_July_2012 25] [http://2012.igem.org/Team:Technion/26_July_2012 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_July_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_July_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/Team:Technion/29_July_2012 29]
[http://2012.igem.org/Team:Technion/30_July_2012 30] [http://2012.igem.org/Team:Technion/31_July_2012 31]
August
MTWTFSS
    [http://2012.igem.org/Team:Technion/1_August_2012 1] [http://2012.igem.org/Team:Technion/2_August_2012 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_August_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/Team:Technion/4_August_2012 4] [http://2012.igem.org/Team:Technion/5_August_2012 5]
[http://2012.igem.org/Team:Technion/6_August_2012 6] [http://2012.igem.org/Team:Technion/7_August_2012 7] [http://2012.igem.org/Team:Technion/8_August_2012 8] [http://2012.igem.org/Team:Technion/9_August_2012 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_August_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_August_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/Team:Technion/12_August_2012 12]
[http://2012.igem.org/Team:Technion/13_August_2012 13] [http://2012.igem.org/Team:Technion/14_August_2012 14] [http://2012.igem.org/Team:Technion/15_August_2012 15] [http://2012.igem.org/Team:Technion/16_August_2012 16] [http://2012.igem.org/Team:Technion/17_August_2012 17] [http://2012.igem.org/Team:Technion/18_August_2012 18] [http://2012.igem.org/Team:Technion/19_August_2012 19]
[http://2012.igem.org/Team:Technion/20_August_2012 20] [http://2012.igem.org/Team:Technion/21_August_2012 21] [http://2012.igem.org/Team:Technion/22_August_2012 22] [http://2012.igem.org/Team:Technion/23_August_2012 23] [http://2012.igem.org/Team:Technion/24_August_2012 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_August_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/Team:Technion/26_August_2012 26]
[http://2012.igem.org/Team:Technion/27_August_2012 27] [http://2012.igem.org/Team:Technion/28_August_2012 28] [http://2012.igem.org/Team:Technion/29_August_2012 29] [http://2012.igem.org/Team:Technion/30_August_2012 30] [http://2012.igem.org/Team:Technion/31_August_2012 31]
September
MTWTFSS
          [http://2012.igem.org/Team:Technion/1_September_2012 1] [http://2012.igem.org/Team:Technion/2_September_2012 2]
[http://2012.igem.org/Team:Technion/3_September_2012 3] [http://2012.igem.org/Team:Technion/4_September_2012 4] [http://2012.igem.org/Team:Technion/5_September_2012 5] [http://2012.igem.org/Team:Technion/6_September_2012 6] [http://2012.igem.org/Team:Technion/7_September_2012 7] [http://2012.igem.org/Team:Technion/8_September_2012 8] [http://2012.igem.org/Team:Technion/9_September_2012 9]
[http://2012.igem.org/Team:Technion/10_September_2012 10] [http://2012.igem.org/Team:Technion/11_September_2012 11] [http://2012.igem.org/Team:Technion/12_September_2012 12] [http://2012.igem.org/Team:Technion/13_September_2012 13] [http://2012.igem.org/Team:Technion/14_September_2012 14] [http://2012.igem.org/Team:Technion/15_September_2012 15] [http://2012.igem.org/Team:Technion/16_September_2012 16]
[http://2012.igem.org/Team:Technion/17_September_2012 17] [http://2012.igem.org/Team:Technion/18_September_2012 18] [http://2012.igem.org/Team:Technion/19_September_2012 19] [http://2012.igem.org/Team:Technion/20_September_2012 20] [http://2012.igem.org/Team:Technion/21_September_2012 21] [http://2012.igem.org/Team:Technion/22_September_2012 22] [http://2012.igem.org/Team:Technion/23_September_2012 23]
[http://2012.igem.org/Team:Technion/24_September_2012 24] [http://2012.igem.org/Team:Technion/25_September_2012 25] [http://2012.igem.org/Team:Technion/26_September_2012 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_September_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_September_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_September_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_September_2012&preload=Team:Technion/Template:NewDate&action=edit 30]
October
MTWTFSS
[http://2012.igem.org/wiki/index.php?title=Team:Technion/1_October_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_October_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_October_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_October_2012&preload=Team:Technion/Template:NewDate&action=edit 4] [http://2012.igem.org/wiki/index.php?title=Team:Technion/5_October_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_October_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_October_2012&preload=Team:Technion/Template:NewDate&action=edit 7]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/8_October_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_October_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_October_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_October_2012&preload=Team:Technion/Template:NewDate&action=edit 11] [http://2012.igem.org/wiki/index.php?title=Team:Technion/12_October_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_October_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_October_2012&preload=Team:Technion/Template:NewDate&action=edit 14]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/15_October_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_October_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_October_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_October_2012&preload=Team:Technion/Template:NewDate&action=edit 18] [http://2012.igem.org/wiki/index.php?title=Team:Technion/19_October_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_October_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_October_2012&preload=Team:Technion/Template:NewDate&action=edit 21]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/22_October_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_October_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_October_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_October_2012&preload=Team:Technion/Template:NewDate&action=edit 25] [http://2012.igem.org/wiki/index.php?title=Team:Technion/26_October_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_October_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_October_2012&preload=Team:Technion/Template:NewDate&action=edit 28]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/29_October_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_October_2012&preload=Team:Technion/Template:NewDate&action=edit 30] [http://2012.igem.org/wiki/index.php?title=Team:Technion/31_October_2012&preload=Team:Technion/Template:NewDate&action=edit 31]
November
MTWTFSS
      [http://2012.igem.org/wiki/index.php?title=Team:Technion/1_November_2012&preload=Team:Technion/Template:NewDate&action=edit 1] [http://2012.igem.org/wiki/index.php?title=Team:Technion/2_November_2012&preload=Team:Technion/Template:NewDate&action=edit 2] [http://2012.igem.org/wiki/index.php?title=Team:Technion/3_November_2012&preload=Team:Technion/Template:NewDate&action=edit 3] [http://2012.igem.org/wiki/index.php?title=Team:Technion/4_November_2012&preload=Team:Technion/Template:NewDate&action=edit 4]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/5_November_2012&preload=Team:Technion/Template:NewDate&action=edit 5] [http://2012.igem.org/wiki/index.php?title=Team:Technion/6_November_2012&preload=Team:Technion/Template:NewDate&action=edit 6] [http://2012.igem.org/wiki/index.php?title=Team:Technion/7_November_2012&preload=Team:Technion/Template:NewDate&action=edit 7] [http://2012.igem.org/wiki/index.php?title=Team:Technion/8_November_2012&preload=Team:Technion/Template:NewDate&action=edit 8] [http://2012.igem.org/wiki/index.php?title=Team:Technion/9_November_2012&preload=Team:Technion/Template:NewDate&action=edit 9] [http://2012.igem.org/wiki/index.php?title=Team:Technion/10_November_2012&preload=Team:Technion/Template:NewDate&action=edit 10] [http://2012.igem.org/wiki/index.php?title=Team:Technion/11_November_2012&preload=Team:Technion/Template:NewDate&action=edit 11]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/12_November_2012&preload=Team:Technion/Template:NewDate&action=edit 12] [http://2012.igem.org/wiki/index.php?title=Team:Technion/13_November_2012&preload=Team:Technion/Template:NewDate&action=edit 13] [http://2012.igem.org/wiki/index.php?title=Team:Technion/14_November_2012&preload=Team:Technion/Template:NewDate&action=edit 14] [http://2012.igem.org/wiki/index.php?title=Team:Technion/15_November_2012&preload=Team:Technion/Template:NewDate&action=edit 15] [http://2012.igem.org/wiki/index.php?title=Team:Technion/16_November_2012&preload=Team:Technion/Template:NewDate&action=edit 16] [http://2012.igem.org/wiki/index.php?title=Team:Technion/17_November_2012&preload=Team:Technion/Template:NewDate&action=edit 17] [http://2012.igem.org/wiki/index.php?title=Team:Technion/18_November_2012&preload=Team:Technion/Template:NewDate&action=edit 18]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/19_November_2012&preload=Team:Technion/Template:NewDate&action=edit 19] [http://2012.igem.org/wiki/index.php?title=Team:Technion/20_November_2012&preload=Team:Technion/Template:NewDate&action=edit 20] [http://2012.igem.org/wiki/index.php?title=Team:Technion/21_November_2012&preload=Team:Technion/Template:NewDate&action=edit 21] [http://2012.igem.org/wiki/index.php?title=Team:Technion/22_November_2012&preload=Team:Technion/Template:NewDate&action=edit 22] [http://2012.igem.org/wiki/index.php?title=Team:Technion/23_November_2012&preload=Team:Technion/Template:NewDate&action=edit 23] [http://2012.igem.org/wiki/index.php?title=Team:Technion/24_November_2012&preload=Team:Technion/Template:NewDate&action=edit 24] [http://2012.igem.org/wiki/index.php?title=Team:Technion/25_November_2012&preload=Team:Technion/Template:NewDate&action=edit 25]
[http://2012.igem.org/wiki/index.php?title=Team:Technion/26_November_2012&preload=Team:Technion/Template:NewDate&action=edit 26] [http://2012.igem.org/wiki/index.php?title=Team:Technion/27_November_2012&preload=Team:Technion/Template:NewDate&action=edit 27] [http://2012.igem.org/wiki/index.php?title=Team:Technion/28_November_2012&preload=Team:Technion/Template:NewDate&action=edit 28] [http://2012.igem.org/wiki/index.php?title=Team:Technion/29_November_2012&preload=Team:Technion/Template:NewDate&action=edit 29] [http://2012.igem.org/wiki/index.php?title=Team:Technion/30_November_2012&preload=Team:Technion/Template:NewDate&action=edit 30]


Ilya

- Glycerol stock of R0062+Fus1 in pSB1C3 and pSB3C5 and of Fus2 in pSB3C5.
- Restriction of R0062+Fus1 in pSB1C3 and pSB3C5 and of Fus2 in pSB1C3 and pSB3C5 PCR products with PacI.
- Gel purification of all restriction products.
- Ligation of all 4 reactions overnight in 16 degrees.

Inbal

-Returning on the PCR from yesterday, But with different Tm's with the same primers (for pSB1C3 & pPROLAR separately) . The results: still not successful- there weren't any bands closed to 2700 bp.
The conclusion- We (Shahar, Evgeni, Rachel and I) need to plan new primers for the RNAP's PCR.

Asaf

-I ran the PCR products from yesterday on gel agarose to check what I got, and I got the expected bands (500 bp).
-I planned the PCR reaction for extracting the different RNA polymerase genes for the different plasmids with the
appropriate overhangs (T7*,N4,T3,K1F,SP6,T7 w.t).

Hila

- restriction reaction with XbaI + PstI (HF) to plasmid pSB1C3.
- 1% gel for plasmid backbone.
today the gel look good!!!

gel purification for the MCS insert and the pSB1C3 cut backbone.
CIP for the plasmids back bone.

Lior

Today me and Noa did a second PCR run for Chris's plasmids- k1f, T3, pN4, sp6. only the k1f came out good. we'll change the Tm for better result in the future PCR programs.
we've done transformation for the xyIE and ALP in Top10 cells and plated them on CM plate.

Noa

Evgeni

Shahar

trying to do the PCR from yesterday again, But with different Tm's with the same primers (for pSB1C3 & pPROLAR separately) . The results: fail. there weren't any bands closed to 2700 bp.
conclusion- there are problems with the priners, and we need to plan new primers for the RNAP's PCR.

Rachel