Team:LMU-Munich

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|<font size="5">'''Opening ''Bacillus subtilis'' and its unique biology to the iGEM world'''</font>
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==Project Abstract==
 
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Our project will combine the idea of biological "beads" with the production of endospores in the gram-positive bacteria ''Bacillus subtilis''. Our goal is to induce the production of specific proteins on the outer surface of ''B. subtilis'' endospores, and knock out spore germination-inducing genes such that the spores can be used as beads, without the ability to germinate.
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|[[File:NEXT.png|100px|link=Team:LMU-Munich/Abstract]]
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|'''Bead'''zillus - For a guided tour through the most important information about our project and results, klick [[Team:LMU-Munich/Abstract|here]].
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|[[File:LMU_Why_BS.png|100px|link=Team:LMU-Munich/Why_Beadzillus]]
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|Why is there a need for our Project [[Team:LMU-Munich/Why_Beadzillus|'''Bead'''zillus]]?
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|[[File:LMU_The_solution.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|Why we choose to work with [[Team:LMU-Munich/Bacillus_Introduction|''Bacillus subtilis'']].
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|[[File:Requirements.png|95px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|To carry out our project we first to had to create a [[Team:LMU-Munich/Bacillus_BioBricks|<b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox]] and had to think over important [[Team:LMU-Munich/Germination_Stop|security issues]].
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|[[File:4-Sporobeads.png|95px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|Finally, we succeeded in displaying proteins on endospores. Our [[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]].
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|[[File:LMU 5-Application.png|90px|link=Team:LMU-Munich/Application]]
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|With our '''Sporo'''beads a lot of [[Team:LMU-Munich/Application|applications]] are imaginable.
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''B. subtilis'' spores are particularly suited to be used in industrial-type applications as "beads" due to their resistance to extreme heat, cold, to desiccation and disinfectants, and even to radiation.
 
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Each ''B. subtilis'' cell produces only one endospore -- in addition to developing our altered strain, we must work to ensure a dependable supply, since we will be removing one of their means of reproduction.
 
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'''Check out our poster:'''
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==='''Bead'''zillus: Fundamental BioBricks for ''Bacillus subtilis'' and spores as a platform for protein display===
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[[File:LMU Project Poster.jpg|200px|center]]
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==Team Background==
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We chose to work with [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction <i>Bacillus subtilis</i>] to set new horizons and offer tools for this model organism to the <i>Escherichia coli</i>-dominated world of iGEM. Therefore, we created a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks <b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox] (<b>B<sup>4</sup></b>) composed of reporter genes, defined promoters, as well as reporter, expression, and empty vectors in BioBrick standard. <i>B. subtilis</i> naturally produces stress-resistant endospores which can germinate in response to suitable environmental conditions. To highlight this unique feature using the <b>B<sup>4</sup></b>, we developed [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins <b>Sporo</b>beads]. These are spores displaying fusion proteins on their surface. As a proof of principle, we fused GFP to the outermost layer. Expanding this idea, we designed a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks/Sporovector <b>Sporo</b>vector] to easily create any <b>Sporo</b>bead imaginable. Because the <b>Sporo</b>beads must be biologically safe and stable vehicles, we [https://2012.igem.org/Team:LMU-Munich/Germination_Stop prevented germination] by knocking out involved genes and developed a [https://2012.igem.org/Team:LMU-Munich/Germination_Stop <b>Suicide</b>switch] turned on in case of germination. With the project <b>Bead</b>zillus, our team demonstrates the powerful nature of <i>B. subtilis</i>.
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[[Image:LMU-Munich_team.png|400px|center]]
 
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====Team LMU Munich 2012====
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We are the iGEM team of the Ludwig-Maximilians-University (LMU) in Munich. The team consists of 10 dedicated members: 7 students and our 3 advisors. Visit our [https://2012.igem.org/Team:LMU-Munich/Team Team] page for more Details.
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We are the iGEM team of the Ludwig-Maximilians-University (LMU) in Munich. The team consists of 10 dedicated members: 7 students and our 3 advisors.
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<a href="http://www.en.uni-muenchen.de/index.html">
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<a href="http://www.biologie.uni-muenchen.de/index.html">
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<img src="https://static.igem.org/mediawiki/2012/4/48/Biozentrum_LMU.jpg" height="170" border=0 /></a></html>
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[[Image:LMU-Munich_logo.png|200px|center]]
 
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[[File:Facebook logo amsterdam.png|70px|left|link=https://www.facebook.com/igem.lmu]] Visit us on facebook
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Visit us on facebook
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http://www.facebook.com/igem.lmu
 
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[[File:Exe12_Youtubelogo.png|65px|left]]
 
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Check out our videos!
 
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http://www.youtube.com/user/igemLMUmunich
 
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[[File:Exe12_Youtubelogo.png|65px|left|link=http://www.youtube.com/user/igemLMUmunich]] Check out our videos!
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| [[Team:LMU-Munich/Team|Find out who we are!]]     
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====Our Gold Sponsors====
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<a href="https://2012.igem.org/Team:LMU-Munich/Sponsors">
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Latest revision as of 13:46, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

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